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Literature summary for 3.2.2.4 extracted from
- A mutant AMP nucleosidase. Purification, properties and in vivo turnover of the protein (1981), J. Biol. Chem., 256, 12823-12829.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| MgATP2- | mutant AMP nucleosidase | Azotobacter vinelandii |  |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | treatment with nitrosoguanidine leads to mutants defective in degrading AMP | Azotobacter vinelandii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Formycin monophosphate | mutant AMP nucleosidase | Azotobacter vinelandii | |
| phosphate | mutant AMP nucleosidase | Azotobacter vinelandii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.16 | - |
AMP | mutant AMP nucleosidase | Azotobacter vinelandii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
6 * 54000, gel filtration | Azotobacter vinelandii |
| 320000 | - |
gel filtration | Azotobacter vinelandii |
| 330000 | - |
mutant AMP nucleosidase, gel filtration | Azotobacter vinelandii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| AMP + H2O | Azotobacter vinelandii | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Azotobacter vinelandii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| mutant AMP nucleosidase | Azotobacter vinelandii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.3 | - |
mutant enzyme | Azotobacter vinelandii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| AMP + H2O | - |
Azotobacter vinelandii | adenine + ribose 5-phosphate | - |
? | |
| AMP + H2O | - |
Azotobacter vinelandii | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 54000, gel filtration | Azotobacter vinelandii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
AMP | mutant enzyme | Azotobacter vinelandii | |
| 62 | - |
AMP | - |
Azotobacter vinelandii | |
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