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Literature summary for 3.2.2.31 extracted from

  • Manuel, R.C.; Hitomi, K.; Arvai, A.S.; House, P.G.; Kurtz, A.J.; Dodson, M.L.; McCullough, A.K.; Tainer, J.A.; Lloyd, R.S.
    Reaction intermediates in the catalytic mechanism of Escherichia coli MutY DNA glycosylase (2004), J. Biol. Chem., 279, 46930-46939 .
    View publication on PubMed
Search for more literature for 3.2.2.31

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli CC104 cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
D138C the mutation completely inhibits the ability of the enzyme to release guanine from DNA containing a guanine:7,8-dihydro-8-oxo-2'-deoxyguanosine mismatch Escherichia coli
E37C the mutation still catalyzes beta- and delta-elimination reactions and can be trapped as covalent enzyme-DNA intermediates by chemical reduction Escherichia coli
K142A the mutation still catalyzes beta- and delta-elimination reactions and can be trapped as covalent enzyme-DNA intermediates by chemical reduction Escherichia coli
K20A the mutation still catalyzes beta- and delta-elimination reactions and can be trapped as covalent enzyme-DNA intermediates by chemical reduction Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA containing a 7,8-dihydro-8-oxo-2'-deoxyguanosine:adenine mismatch + H2O Escherichia coli
-
 ?
-
 ?
DNA containing a guanine:adenine mismatch + H2O Escherichia coli
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA containing a 7,8-dihydro-8-oxo-2'-deoxyguanosine:adenine mismatch + H2O
-
 Escherichia coli ?
-
 ?
DNA containing a 7,8-dihydro-8-oxo-2'-deoxyguanosine:guanine mismatch + H2O
-
 Escherichia coli ?
-
 ?
DNA containing a guanine:adenine mismatch + H2O
-
 Escherichia coli ?
-
 ?
additional information no activity with DNA containing a guanine:guanine mismatch Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
mutY
-
 Escherichia coli