Protein Variants | Comment | Organism |
---|---|---|
G253A | when 2'-deoxy-2'-fluoroadenosine in duplex paired with guanine or 7,8-dihydro-8-oxo-2'-deoxyguanosine is used, the mutant shows reduced binding affinity. Additionally, compromised glycosylase activity of the mutant enzyme is observed using the nonoptimal guanine:adenine substrate, or at low reaction temperatures | Escherichia coli |
G253D | the mutant has a reduced adenine glycosylase activity and affinity for substrate analogues compared to the wild type enzyme | Escherichia coli |
Y82C | the mutant has a reduced adenine glycosylase activity and affinity for substrate analogues compared to the wild type enzyme. Additionally, compromised glycosylase activity of the mutant enzyme is observed using the nonoptimal guanine:adenine substrate, or at low reaction temperatures | Escherichia coli |
Y82F | when 2-deoxy-2-fluoroadenosine in duplex paired with guanine or 7,8-dihydro-8-oxo-2'-deoxyguanosine is used, the mutant shows reduced binding affinity. Additionally, compromised glycosylase activity of the mutant enzyme is observed using the nonoptimal guanine:adenine substrate, or at low reaction temperatures | Escherichia coli |
Y82L | the mutant is unable to discriminate between guanine and 7,8-dihydro-8-oxo-2'-deoxyguanosine when paired with 2'-deoxy-2'-fluoroadenosine | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA containing a 7,8-dihydro-8-oxo-2'-deoxyguanosine:adenine mispair + H2O | Escherichia coli | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and HiTrap heparin column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA containing a 7,8-dihydro-8-oxo-2'-deoxyguanosine:adenine mispair + H2O | - |
Escherichia coli | ? | - |
? | |
DNA containing a guanine:adenine mispair + H2O | - |
Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
adenine glycosylase | - |
Escherichia coli |
mutY | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme plays an important role in preventing 7,8-dihydro-8-oxo-2'-deoxyguanosine-associated mutations by removing adenines misincorporated opposite 7,8-dihydro-8-oxo-2'-deoxyguanosine lesions during DNA replication | Escherichia coli |