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Literature summary for 3.2.2.31 extracted from

  • Messick, T.E.; Chmiel, N.H.; Golinelli, M.P.; Langer, M.R.; Joshua-Tor, L.; David, S.S.
    Noncysteinyl coordination to the [4Fe-4S]2+ cluster of the DNA repair adenine glycosylase MutY introduced via site-directed mutagenesis. Structural characterization of an unusual histidinyl-coordinated cluster (2002), Biochemistry, 41, 3931-3942 .
    View publication on PubMed
Search for more literature for 3.2.2.31

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant enzyme C199H, hanging drop vapor diffusion method, using 1.6-1.8 M ammonium sulfate and 100 mM Tris-HCl, pH 8.5 Escherichia coli

Protein Variants

Protein Variants Comment Organism
C199H the mutant shows wild type activity Escherichia coli
C199S the mutant shows wild type activity Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA containing a 7,8-dihydro-8-oxo-2'-deoxyguanosine:adenine mismatch + H2O Escherichia coli
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P17802
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA containing a 7,8-dihydro-8-oxo-2'-deoxyguanosine:adenine mismatch + H2O
-
 Escherichia coli ?
-
 ?
DNA containing a guanine:adenine mismatch + H2O
-
 Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
DNA repair adenine glycosylase
-
 Escherichia coli
mutY
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
[4Fe-4S]-center
-
 Escherichia coli

General Information

General Information Comment Organism
physiological function the enzyme prevents DNA mutations caused by the misincorporation of adenine opposite 7,8-dihydro-8-oxo-2'-deoxyguanosine by catalyzing the deglycosylation of the aberrant adenine Escherichia coli