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Literature summary for 3.2.2.31 extracted from

  • Williams, S.D.; David, S.S.
    A single engineered point mutation in the adenine glycosylase MutY confers bifunctional glycosylase/AP lyase activity (2000), Biochemistry, 39, 10098-10109 .
    View publication on PubMed
Search for more literature for 3.2.2.31

Protein Variants

Protein Variants Comment Organism
K142A the mutant shows slightly reduced activity compared to the wild type enzyme Escherichia coli
S120K the mutant with bifunctional glycosylase/AP lyase activity is capable of catalyzing DNA strand scission at a rate equivalent to that of adenine excision for both guanine:adenine and 7,8-dihydro-8-oxo-2'-deoxyguanosine:adenine mispair substrates Escherichia coli
S120K/K142A the mutant with bifunctional glycosylase/AP lyase activity is capable of catalyzing DNA strand scission at a rate equivalent to that of adenine excision for both guanine:adenine and 7,8-dihydro-8-oxo-2'-deoxyguanosine:adenine mispair substrates Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA containing a 7,8-dihydro-8-oxo-2'-deoxyguanosine:adenine mismatch + H2O Escherichia coli
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P17802
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA containing a 7,8-dihydro-8-oxo-2'-deoxyguanosine:adenine mismatch + H2O
-
 Escherichia coli ?
-
 ?
DNA containing a guanine:adenine mismatch + H2O
-
 Escherichia coli ?
-
 ?
additional information the enzyme has no activity with 2'-deoxyaristeromycin, 2'-deoxy-2'-fluoroadenosine, and 2'-deoxyformycin A Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
mutY
-
 Escherichia coli