Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.2.30 extracted from

  • Li, X.; Apel, D.; Gaynor, E.C.; Tanner, M.E.
    5'-methylthioadenosine nucleosidase is implicated in playing a key role in a modified futalosine pathway for menaquinone biosynthesis in Campylobacter jejuni (2011), J. Biol. Chem., 286, 19392-19398.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as a His-tagged protein Campylobacter jejuni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
6-amino-6-deoxyfutalosine pH 7, 25°C Campylobacter jejuni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-amino-6-deoxyfutalosine + H2O Campylobacter jejuni the enzyme is part of a modified futalosine pathway for menaquinone biosynthesis dehypoxanthine futalosine + adenine
-
?
6-amino-6-deoxyfutalosine + H2O Campylobacter jejuni NCTC 11168 the enzyme is part of a modified futalosine pathway for menaquinone biosynthesis dehypoxanthine futalosine + adenine
-
?

Organism

Organism UniProt Comment Textmining
Campylobacter jejuni Q0PC20
-
-
Campylobacter jejuni NCTC 11168 Q0PC20
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Campylobacter jejuni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-amino-6-deoxyfutalosine + H2O the enzyme is part of a modified futalosine pathway for menaquinone biosynthesis Campylobacter jejuni dehypoxanthine futalosine + adenine
-
?
6-amino-6-deoxyfutalosine + H2O 6-amino-6-deoxyfutalosine i.e. 3-[3-[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]propanoyl]benzoate. The activity with futalosine ist 2% compared to the activity with 6-amino-6-deoxyfutalosine. The enzyme also has the activity of EC 3.2.2.9, adenosylhomocysteine nucleosidase Campylobacter jejuni dehypoxanthine futalosine + adenine dehypoxanthine futalosine i.e. 3-[3-[(2R,3S,4R)-3,4,5-trihydroxytetrahydrofuran-2-yl]propanoyl]benzoate ?
6-amino-6-deoxyfutalosine + H2O the enzyme is part of a modified futalosine pathway for menaquinone biosynthesis Campylobacter jejuni NCTC 11168 dehypoxanthine futalosine + adenine
-
?
6-amino-6-deoxyfutalosine + H2O 6-amino-6-deoxyfutalosine i.e. 3-[3-[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]propanoyl]benzoate. The activity with futalosine ist 2% compared to the activity with 6-amino-6-deoxyfutalosine. The enzyme also has the activity of EC 3.2.2.9, adenosylhomocysteine nucleosidase Campylobacter jejuni NCTC 11168 dehypoxanthine futalosine + adenine dehypoxanthine futalosine i.e. 3-[3-[(2R,3S,4R)-3,4,5-trihydroxytetrahydrofuran-2-yl]propanoyl]benzoate ?

Synonyms

Synonyms Comment Organism
AFL nucleosidase
-
Campylobacter jejuni
aminofutalosine nucleosidase
-
Campylobacter jejuni
Cj0117
-
Campylobacter jejuni
methylthioadenosine nucleosidase
-
Campylobacter jejuni
MqnB
-
Campylobacter jejuni

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Campylobacter jejuni

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.53
-
6-amino-6-deoxyfutalosine pH 7, 25°C Campylobacter jejuni

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Campylobacter jejuni

General Information

General Information Comment Organism
physiological function the enzyme is part of a modified futalosine pathway for menaquinone biosynthesis Campylobacter jejuni