Protein Variants | Comment | Organism |
---|---|---|
H254A | sited-directed mutagenesis, active site mutant, 30fold decreased Vmax and unaltered Km compared to the wild-type enzyme | Saccharomyces cerevisiae |
additional information | construction of urh115 disruption mutant strains | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-fluorocytidine | noncompetitive | Saccharomyces cerevisiae | |
5-fluorouridine | competitive | Saccharomyces cerevisiae | |
cytidine | noncompetitive | Saccharomyces cerevisiae | |
deoxycytidine | competitive | Saccharomyces cerevisiae | |
Inosine | noncompetitive | Saccharomyces cerevisiae | |
ribosylthymine | competitive | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Saccharomyces cerevisiae | physiological function, investigation of metabolic fluxes in several mutants impaired in the pyrimidine salvage pathway, regulation, overview | ? | - |
? | |
uridine + H2O | Saccharomyces cerevisiae | enzyme is important for recycling, via the salvage pathway, pyrimidine deoxy- and ribonucleosides into uracil required for the growth of strains lacking de novo pyrimidine synthesis pathway | D-ribose + uracil | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q04179 | wild-type strain MATaa and mutant derivatives, gene URH1 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
uridine + H2O = D-ribose + uracil | substrate binding site, active site structure, His254 is important, catalytic mechanism | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-fluorocytidine + H2O | - |
Saccharomyces cerevisiae | D-ribose + 5-fluorocytosine | - |
? | |
5-fluorouridine + H2O | - |
Saccharomyces cerevisiae | D-ribose + 5-fluorouracil | - |
? | |
cytidine + H2O | - |
Saccharomyces cerevisiae | D-ribose + cytosine | - |
? | |
deoxycytidine + H2O | - |
Saccharomyces cerevisiae | D-deoxyribose + cytosine | - |
? | |
additional information | physiological function, investigation of metabolic fluxes in several mutants impaired in the pyrimidine salvage pathway, regulation, overview | Saccharomyces cerevisiae | ? | - |
? | |
additional information | substrate specificity, enzyme possesses no phosphorylase activity | Saccharomyces cerevisiae | ? | - |
? | |
uridine + H2O | - |
Saccharomyces cerevisiae | D-ribose + uracil | - |
? | |
uridine + H2O | enzyme is important for recycling, via the salvage pathway, pyrimidine deoxy- and ribonucleosides into uracil required for the growth of strains lacking de novo pyrimidine synthesis pathway | Saccharomyces cerevisiae | D-ribose + uracil | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Urh1p | - |
Saccharomyces cerevisiae |
uridine/cytidine hydrolase | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
5-fluorouridine | pH 7.5, 37°C, wild-type enzyme, versus uridine | Saccharomyces cerevisiae | |
2 | - |
cytidine | pH 7.5, 37°C, wild-type enzyme, versus uridine | Saccharomyces cerevisiae | |
2 | - |
ribosylthymine | pH 7.5, 37°C, wild-type enzyme, versus uridine | Saccharomyces cerevisiae | |
3 | - |
Inosine | pH 7.5, 37°C, wild-type enzyme, versus uridine | Saccharomyces cerevisiae | |
3 | - |
deoxycytidine | pH 7.5, 37°C, wild-type enzyme, versus uridine | Saccharomyces cerevisiae | |
3 | - |
5-fluorocytidine | pH 7.5, 37°C, wild-type enzyme, versus uridine | Saccharomyces cerevisiae |