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Literature summary for 3.2.2.3 extracted from

  • Kurtz, J.E.; Exinger, F.; Erbs, P.; Jund, R.
    The URH1 uridine ribohydrolase of Saccharomyces cerevisiae (2002), Curr. Genet., 41, 132-141.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
H254A sited-directed mutagenesis, active site mutant, 30fold decreased Vmax and unaltered Km compared to the wild-type enzyme Saccharomyces cerevisiae
additional information construction of urh115 disruption mutant strains Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
5-fluorocytidine noncompetitive Saccharomyces cerevisiae
5-fluorouridine competitive Saccharomyces cerevisiae
cytidine noncompetitive Saccharomyces cerevisiae
deoxycytidine competitive Saccharomyces cerevisiae
Inosine noncompetitive Saccharomyces cerevisiae
ribosylthymine competitive Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Saccharomyces cerevisiae physiological function, investigation of metabolic fluxes in several mutants impaired in the pyrimidine salvage pathway, regulation, overview ?
-
?
uridine + H2O Saccharomyces cerevisiae enzyme is important for recycling, via the salvage pathway, pyrimidine deoxy- and ribonucleosides into uracil required for the growth of strains lacking de novo pyrimidine synthesis pathway D-ribose + uracil
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae Q04179 wild-type strain MATaa and mutant derivatives, gene URH1
-

Reaction

Reaction Comment Organism Reaction ID
uridine + H2O = D-ribose + uracil substrate binding site, active site structure, His254 is important, catalytic mechanism Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-fluorocytidine + H2O
-
Saccharomyces cerevisiae D-ribose + 5-fluorocytosine
-
?
5-fluorouridine + H2O
-
Saccharomyces cerevisiae D-ribose + 5-fluorouracil
-
?
cytidine + H2O
-
Saccharomyces cerevisiae D-ribose + cytosine
-
?
deoxycytidine + H2O
-
Saccharomyces cerevisiae D-deoxyribose + cytosine
-
?
additional information physiological function, investigation of metabolic fluxes in several mutants impaired in the pyrimidine salvage pathway, regulation, overview Saccharomyces cerevisiae ?
-
?
additional information substrate specificity, enzyme possesses no phosphorylase activity Saccharomyces cerevisiae ?
-
?
uridine + H2O
-
Saccharomyces cerevisiae D-ribose + uracil
-
?
uridine + H2O enzyme is important for recycling, via the salvage pathway, pyrimidine deoxy- and ribonucleosides into uracil required for the growth of strains lacking de novo pyrimidine synthesis pathway Saccharomyces cerevisiae D-ribose + uracil
-
?

Synonyms

Synonyms Comment Organism
Urh1p
-
Saccharomyces cerevisiae
uridine/cytidine hydrolase
-
Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.6
-
5-fluorouridine pH 7.5, 37°C, wild-type enzyme, versus uridine Saccharomyces cerevisiae
2
-
cytidine pH 7.5, 37°C, wild-type enzyme, versus uridine Saccharomyces cerevisiae
2
-
ribosylthymine pH 7.5, 37°C, wild-type enzyme, versus uridine Saccharomyces cerevisiae
3
-
Inosine pH 7.5, 37°C, wild-type enzyme, versus uridine Saccharomyces cerevisiae
3
-
deoxycytidine pH 7.5, 37°C, wild-type enzyme, versus uridine Saccharomyces cerevisiae
3
-
5-fluorocytidine pH 7.5, 37°C, wild-type enzyme, versus uridine Saccharomyces cerevisiae