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Literature summary for 3.2.2.3 extracted from
- Uridine nucleosidase from yeast (1978), Methods Enzymol., 51, 290-296.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Saccharomyces cerevisiae |  |
| CdCl2 | - |
Saccharomyces cerevisiae | |
| Co2+ | CoCl2 | Saccharomyces cerevisiae | |
| CuCl2 | - |
Saccharomyces cerevisiae | |
| D-ribose | competitive | Saccharomyces cerevisiae | |
| EDTA | - |
Saccharomyces cerevisiae | |
| glucose 6-phosphate | competitive | Saccharomyces cerevisiae | |
| MnCl2 | - |
Saccharomyces cerevisiae | |
| NaCN | - |
Saccharomyces cerevisiae | |
| NiCl2 | - |
Saccharomyces cerevisiae | |
| ribose 5-phosphate | - |
Saccharomyces cerevisiae | |
| ribosylthymine | competitive | Saccharomyces cerevisiae | |
| ribulose 5-phosphate | - |
Saccharomyces cerevisiae | |
| ZnCl2 | - |
Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.86 | - |
uridine | - |
Saccharomyces cerevisiae | |
| 1.66 | - |
5-methyluridine | - |
Saccharomyces cerevisiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | contains 1 gatom of Cu2+ per enzyme molecule | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16500 | - |
2 * 16500, SDS-PAGE | Saccharomyces cerevisiae |
| 32500 | - |
gel filtration | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.56 | - |
- |
Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for several months | Saccharomyces cerevisiae |
| 4°C, 100 mM phosphate buffer, pH 7.2, 50 mM Tris-HCl, pH 7.0, stable for up to 15 days | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-methyluridine + H2O | - |
Saccharomyces cerevisiae | 5-methyluracil + D-ribose | - |
? | |
| uridine + H2O | - |
Saccharomyces cerevisiae | uracil + D-ribose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 16500, SDS-PAGE | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.1 | - |
- |
Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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