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Literature summary for 3.2.2.29 extracted from

  • Hashimoto, H.; Zhang, X.; Cheng, X.
    Activity and crystal structure of human thymine DNA glycosylase mutant N140A with 5-carboxylcytosine DNA at low pH (2013), DNA Repair, 12, 535-540.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.29

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant N140A in complex with guanine:5-carboxylcytosine containing DNA, using 30% (w/v) polyethylene glycol 4000, 0.2 M ammonium acetate, 0.1 M sodium acetate, pH 4.6 Homo sapiens

Protein Variants

Protein Variants Comment Organism
N140A the mutant exhibits 1800fold and more than 20000fold reduced activity at pH 6.0 after 24 h for guanine:uracil and after 72 h for guanine:5-carboxylcytosine, respectively Homo sapiens
N140D the mutant exhibits 96fold and 5fold reduced activity at pH 6.0 after 24 h for guanine:uracil and after 72 h for guanine:5-carboxylcytosine, respectively Homo sapiens
N140D/N157D the mutant exhibits no activity for DNA containing guanine:uracil and guanine:5-carboxylcytosine pairs Homo sapiens
N157A the mutant exhibits 6fold and 3fold reduced activity at pH 6.0 after 24 h for guanine:uracil and after 72 h for guanine:5-carboxylcytosine, respectively Homo sapiens
N157D the mutant exhibits 1350fold and 1.1fold reduced activity at pH 6.0 after 24 h for guanine:uracil and after 72 h for guanine:5-carboxylcytosine, respectively Homo sapiens
N157D/N230D the mutant exhibits 135fold reduced activity and no activity at pH 6.0 after 24 h for guanine:uracil and after 72 h for guanine:5-carboxylcytosine, respectively Homo sapiens
N230D the mutant exhibits 32fold and 3fold reduced activity at pH 6.0 after 24 h for guanine:uracil and after 72 h for guanine:5-carboxylcytosine, respectively Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-carboxylcytosine mismatched double-stranded DNA + H2O Homo sapiens
-
 5-carboxylcytosine + double-stranded DNA with abasic site
-
 ?
thymine-mismatched double-stranded DNA + H2O Homo sapiens
-
 thymine + double-stranded DNA with abasic site
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q13569
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-carboxylcytosine mismatched double-stranded DNA + H2O
-
 Homo sapiens 5-carboxylcytosine + double-stranded DNA with abasic site
-
 ?
thymine-mismatched double-stranded DNA + H2O
-
 Homo sapiens thymine + double-stranded DNA with abasic site
-
 ?

Synonyms

Synonyms Comment Organism
TDG
-
 Homo sapiens
thymine DNA glycosylase
-
 Homo sapiens