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Literature summary for 3.2.2.29 extracted from
- Expression and characterization of thymine-DNA glycosylase from Aeropyrum pernix (2010), Protein Expr. Purif., 70, 1-6.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the His-tagged enzyme in Escherichia coli strain BL21 (DE3) | Aeropyrum pernix |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | slight inhibition of the enzymic activity | Aeropyrum pernix |  |
| Co2+ | complete inhibition at 5 mM | Aeropyrum pernix | |
| Cu2+ | complete inhibition at 5 mM | Aeropyrum pernix | |
| Mg2+ | slight inhibition of the enzymic activity | Aeropyrum pernix | |
| Mn2+ | complete inhibition at 5 mM | Aeropyrum pernix | |
| additional information | additional mismatch located at 3' of T/G have less inhibitory effect on the thymine removal than that located at 5' of T/G. Two additional mismatches located at each side of T/G completely inhibit the excision of thymine | Aeropyrum pernix | |
| NaCl | NaCl completely inhibits the thymine removal at 0.4 M | Aeropyrum pernix | |
| Ni2+ | complete inhibition at 5 mM | Aeropyrum pernix | |
| Zn2+ | complete inhibition at 5 mM | Aeropyrum pernix | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aeropyrum pernix | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography | Aeropyrum pernix |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | excision of T:G mismatches in oligonucleotide substrates. Aeropyrum pernix TDG also has a relatively weak DNA glycosylase activity on uracil base, with the following descending order: U/C - U/G = U/T = U/U = U/I = U/AP = U/- - U/A. Additional mismatch located at 3' of T/G have less inhibitory effect on the thymine removal than that located at 5' of T/G. Two additional mismatches located at each side of T/G completely inhibit the excision of thymine | Aeropyrum pernix | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TDG | - |
Aeropyrum pernix |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | 70 | recombinant His-tagged enzyme | Aeropyrum pernix |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 85 | activity range, profile, recombinant His-tagged enzyme, overview | Aeropyrum pernix |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
purified recombinant enzyme, 30 min, completely stable | Aeropyrum pernix |
| 80 | - |
purified recombinant enzyme, 30 min, 50% activity remaining | Aeropyrum pernix |
| 90 | - |
purified recombinant enzyme, 5 min, loss of 60% activity, inactive after 30 min | Aeropyrum pernix |
| 95 | - |
purified recombinant enzyme, 5 min, inactivation | Aeropyrum pernix |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.5 | recombinant His-tagged enzyme | Aeropyrum pernix |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9.5 | activity range, profile, recombinant His-tagged enzyme, overview | Aeropyrum pernix |
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