Literature summary for 3.2.2.29 extracted from

Baba, D.; Maita, N.; Jee, J.G.; Uchimura, Y.; Saitoh, H.; Sugasawa, K.; Hanaoka, F.; Tochio, H.; Hiroaki, H.; Shirakawa, M.
Crystal structure of SUMO-3-modified thymine-DNA glycosylase (2006), J. Mol. Biol., 359, 137-147.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
crystals of SUMO-3TDG are grown in 1.5 M sodium malonate (pH 5.0) at 20°C, by using a streaking technique. Crystal structure of the central region of TDG conjugated to SUMO-3	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
nucleus	-	Homo sapiens	5634	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q13569	-	-

Purification (Commentary)

Purification (Comment)	Organism
cDNA sequence encoding human TDG (residues 112339) is subcloned into a pGEX4T-3 vector. SUMO-3-modified TDG112339 (SUMO-3TDG) is expressed bacterially using an Escherichia coli SUMOylation system by co-transforming the Escherichia coli strain BL21(DE3) with TDG112339/pGEX4T-3 and pT-E1E2S2 protein expression vectors	Homo sapiens

Synonyms

Synonyms	Comment	Organism
TDG	-	Homo sapiens
thymine-DNA glycosylase	-	Homo sapiens

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Release 2023.1 (January 2023)