Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
uracil-mismatched double-stranded DNA + H2O | Homo sapiens | thymine DNA glycosylase may play a backup role to the more efficient general uracil DNA glycosylase | uracil + double-stranded DNA with abasic site | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q13569 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
thymine-mismatched double-stranded DNA + H2O | each molecule of thymine DNA glycosylase removes only one molecule of thymine from DNA containing a G/T mismatch because it binds tightly to the apurinic DNA site left after removal of thymine. The 5'-flanking base pair to G/T mismatches influences the rate of removal of thymine. Thymine DNA glycosylase can also remove thymine from mismatches with S6-methylthioguanine, but, unlike G/T mismatches, a 5'-C-G does not have a striking effect on the rate. Thymine removal is fastest when it is from a G/T mismatch with a 5'-flanking C/G pair, suggesting that the rapid reaction of this substrate involves contacts between the enzyme and oxygen 6 or the N-1 hydrogen of the mismatched guanine as well as the 5'-flanking C/G pair | Homo sapiens | thymine + double-stranded DNA with abasic site | - |
? | |
uracil-mismatched double-stranded DNA + H2O | thymine DNA glycosylase may play a backup role to the more efficient general uracil DNA glycosylase | Homo sapiens | uracil + double-stranded DNA with abasic site | - |
? | |
uracil-mismatched double-stranded DNA + H2O | the glycosylase removes uracil from G/U, C/U, and T/U base pairs faster than it removes thymine from G/T. It can even remove uracil from A/U base pairs, although at a very much lower rate | Homo sapiens | uracil + double-stranded DNA with abasic site | - |
? |
Synonyms | Comment | Organism |
---|---|---|
thymine DNA glycosylase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the 5'-flanking base pair to G/T mismatches influences the rate of removal of thymine from G/T mismatch: kcat values with C/G, T/A, G/C, and A/T as the 5'-base pair are 0.91, 0.023, 0.0046, and 0.0013 per min, respectively. kcat values for removal of thymine from S6-methylthioguanine/T with C/G, T/A, G/C, and A/T as the 5'-base pair are 0.026, 0.018, 0.0017, and 0.0010 min, respectively | Homo sapiens |