Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.2.29 extracted from

  • Waters, T.R.
    Swann, P.F.: Kinetics of the action of thymine DNA glycosylase (1998), J. Biol. Chem., 273, 20007-20014.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
uracil-mismatched double-stranded DNA + H2O Homo sapiens thymine DNA glycosylase may play a backup role to the more efficient general uracil DNA glycosylase uracil + double-stranded DNA with abasic site
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q13569
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
thymine-mismatched double-stranded DNA + H2O each molecule of thymine DNA glycosylase removes only one molecule of thymine from DNA containing a G/T mismatch because it binds tightly to the apurinic DNA site left after removal of thymine. The 5'-flanking base pair to G/T mismatches influences the rate of removal of thymine. Thymine DNA glycosylase can also remove thymine from mismatches with S6-methylthioguanine, but, unlike G/T mismatches, a 5'-C-G does not have a striking effect on the rate. Thymine removal is fastest when it is from a G/T mismatch with a 5'-flanking C/G pair, suggesting that the rapid reaction of this substrate involves contacts between the enzyme and oxygen 6 or the N-1 hydrogen of the mismatched guanine as well as the 5'-flanking C/G pair Homo sapiens thymine + double-stranded DNA with abasic site
-
?
uracil-mismatched double-stranded DNA + H2O thymine DNA glycosylase may play a backup role to the more efficient general uracil DNA glycosylase Homo sapiens uracil + double-stranded DNA with abasic site
-
?
uracil-mismatched double-stranded DNA + H2O the glycosylase removes uracil from G/U, C/U, and T/U base pairs faster than it removes thymine from G/T. It can even remove uracil from A/U base pairs, although at a very much lower rate Homo sapiens uracil + double-stranded DNA with abasic site
-
?

Synonyms

Synonyms Comment Organism
thymine DNA glycosylase
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information the 5'-flanking base pair to G/T mismatches influences the rate of removal of thymine from G/T mismatch: kcat values with C/G, T/A, G/C, and A/T as the 5'-base pair are 0.91, 0.023, 0.0046, and 0.0013 per min, respectively. kcat values for removal of thymine from S6-methylthioguanine/T with C/G, T/A, G/C, and A/T as the 5'-base pair are 0.026, 0.018, 0.0017, and 0.0010 min, respectively Homo sapiens