Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DTT | - |
Monkeypox virus | |
EDTA | - |
Monkeypox virus |
Application | Comment | Organism |
---|---|---|
medicine | inhibition of UNG is a rational therapeutic strategy for infections with poxviruses | Monkeypox virus |
Cloned (Comment) | Organism |
---|---|
gene E4R, expression of the N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Monkeypox virus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4(6)-aminouracil | - |
Monkeypox virus | |
5-Azauracil | strong inhibition | Monkeypox virus | |
5-halogenated uracil analogues | - |
Monkeypox virus | |
MgCl2 | the viral enzyme is strongly inhibited by physiological concentrations of NaCl and MgCl2 | Monkeypox virus | |
additional information | inhibitor screening, no inhibition by uracil | Monkeypox virus | |
NaCl | 10fold inhibition at 10 mM, the viral enzyme is strongly inhibited by physiological concentrations of NaCl and MgCl2 | Monkeypox virus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Monkeypox virus | |
0.000015 | - |
uracil-mismatched double-stranded DNA | pH 7.0, 37°C, excision of a single uracil residue from PS-G, recombinant enzyme | Monkeypox virus | |
0.00003 | - |
uracil-mismatched double-stranded DNA | pH 7.0, 37°C, excision of a single uracil residue from U-G, recombinant enzyme | Monkeypox virus | |
0.001 | - |
uracil-mismatched double-stranded DNA | pH 7.5, 37°C, excision of a single uracil residue from U-A, recombinant enzyme | Monkeypox virus | |
0.001 | - |
uracil-mismatched double-stranded DNA | pH 7.5, 37°C, excision of a single uracil residue from U-G, recombinant enzyme | Monkeypox virus | |
0.0014 | - |
uracil-mismatched double-stranded DNA | pH 7.5, 37°C, excision of a single uracil residue from U-T, recombinant enzyme | Monkeypox virus | |
0.0016 | - |
uracil-mismatched double-stranded DNA | pH 7.5, 37°C, excision of a single uracil residue from U-C, recombinant enzyme | Monkeypox virus | |
0.0017 | - |
uracil-mismatched double-stranded DNA | pH 7.5, 37°C, excision of a single uracil residue from PS-U, recombinant enzyme | Monkeypox virus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27604 | - |
x * 27604, N-terminally His-tagged enzyme, sequence calculation | Monkeypox virus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
uracil-mismatched double-stranded DNA + H2O | Monkeypox virus | under physiological conditions of 60 mM NaCl, pH 7.5, increasing amounts of viral UNG cleave both 45mer U-G and PS-U oligonucleotides. Monkeypox virus, which occurs naturally in Africa, can cause a smallpoxlike disease in humans. The DNA repair protein uracil-DNA glycosylase, UNG, is one of the viral enzymes important for poxvirus pathogenesis, thus inhibition of UNG is a therapeutic strategy, overview | uracil + double-stranded DNA with abasic site | - |
? | |
uracil-mismatched double-stranded DNA + H2O | Monkeypox virus Zaire 1996 | under physiological conditions of 60 mM NaCl, pH 7.5, increasing amounts of viral UNG cleave both 45mer U-G and PS-U oligonucleotides. Monkeypox virus, which occurs naturally in Africa, can cause a smallpoxlike disease in humans. The DNA repair protein uracil-DNA glycosylase, UNG, is one of the viral enzymes important for poxvirus pathogenesis, thus inhibition of UNG is a therapeutic strategy, overview | uracil + double-stranded DNA with abasic site | - |
? | |
uracil-mismatched single-stranded DNA + H2O | Monkeypox virus | Monkeypox virus, which occurs naturally in Africa, can cause a smallpox-like disease in humans. The DNA repair protein uracil-DNA glycosylase, UNG, is one of the viral enzymes important for poxvirus pathogenesis, thus inhibition of UNG is a therapeutic strategy, overview | uracil + single-stranded DNA with abasic site | - |
? | |
uracil-mismatched single-stranded DNA + H2O | Monkeypox virus Zaire 1996 | Monkeypox virus, which occurs naturally in Africa, can cause a smallpox-like disease in humans. The DNA repair protein uracil-DNA glycosylase, UNG, is one of the viral enzymes important for poxvirus pathogenesis, thus inhibition of UNG is a therapeutic strategy, overview | uracil + single-stranded DNA with abasic site | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Monkeypox virus | Q8V4Y4 | gene E4R | - |
Monkeypox virus Zaire 1996 | Q8V4Y4 | gene E4R | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Monkeypox virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
uracil-mismatched double-stranded DNA + H2O | under physiological conditions of 60 mM NaCl, pH 7.5, increasing amounts of viral UNG cleave both 45mer U-G and PS-U oligonucleotides. Monkeypox virus, which occurs naturally in Africa, can cause a smallpoxlike disease in humans. The DNA repair protein uracil-DNA glycosylase, UNG, is one of the viral enzymes important for poxvirus pathogenesis, thus inhibition of UNG is a therapeutic strategy, overview | Monkeypox virus | uracil + double-stranded DNA with abasic site | - |
? | |
uracil-mismatched double-stranded DNA + H2O | cleavage of a duplex oligonucleotide containing a single lesion at a defined position. The viral mpUNG protein excises uracil and prefers the U-G pair over the U-A pair and does not excise oxidized bases | Monkeypox virus | uracil + double-stranded DNA with abasic site | - |
? | |
uracil-mismatched double-stranded DNA + H2O | under physiological conditions of 60 mM NaCl, pH 7.5, increasing amounts of viral UNG cleave both 45mer U-G and PS-U oligonucleotides. Monkeypox virus, which occurs naturally in Africa, can cause a smallpoxlike disease in humans. The DNA repair protein uracil-DNA glycosylase, UNG, is one of the viral enzymes important for poxvirus pathogenesis, thus inhibition of UNG is a therapeutic strategy, overview | Monkeypox virus Zaire 1996 | uracil + double-stranded DNA with abasic site | - |
? | |
uracil-mismatched double-stranded DNA + H2O | cleavage of a duplex oligonucleotide containing a single lesion at a defined position. The viral mpUNG protein excises uracil and prefers the U-G pair over the U-A pair and does not excise oxidized bases | Monkeypox virus Zaire 1996 | uracil + double-stranded DNA with abasic site | - |
? | |
uracil-mismatched single-stranded DNA + H2O | Monkeypox virus, which occurs naturally in Africa, can cause a smallpox-like disease in humans. The DNA repair protein uracil-DNA glycosylase, UNG, is one of the viral enzymes important for poxvirus pathogenesis, thus inhibition of UNG is a therapeutic strategy, overview | Monkeypox virus | uracil + single-stranded DNA with abasic site | - |
? | |
uracil-mismatched single-stranded DNA + H2O | cleavage of a single-stranded oligonucleotide containing a single lesion at a defined position, usage of single-stranded DNA oligonucleotide labeled with a 5'-fluorescein and a 3'-dabsyl. The viral mpUNG protein excises uracil preferentially from single-stranded DNA and does not excise oxidized bases | Monkeypox virus | uracil + single-stranded DNA with abasic site | - |
? | |
uracil-mismatched single-stranded DNA + H2O | Monkeypox virus, which occurs naturally in Africa, can cause a smallpox-like disease in humans. The DNA repair protein uracil-DNA glycosylase, UNG, is one of the viral enzymes important for poxvirus pathogenesis, thus inhibition of UNG is a therapeutic strategy, overview | Monkeypox virus Zaire 1996 | uracil + single-stranded DNA with abasic site | - |
? | |
uracil-mismatched single-stranded DNA + H2O | cleavage of a single-stranded oligonucleotide containing a single lesion at a defined position, usage of single-stranded DNA oligonucleotide labeled with a 5'-fluorescein and a 3'-dabsyl. The viral mpUNG protein excises uracil preferentially from single-stranded DNA and does not excise oxidized bases | Monkeypox virus Zaire 1996 | uracil + single-stranded DNA with abasic site | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 27604, N-terminally His-tagged enzyme, sequence calculation | Monkeypox virus |
Synonyms | Comment | Organism |
---|---|---|
UNG | - |
Monkeypox virus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Monkeypox virus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.042 | - |
uracil-mismatched double-stranded DNA | pH 7.5, 37°C, excision of a single uracil residue from U-A, recombinant enzyme | Monkeypox virus | |
0.3 | - |
uracil-mismatched double-stranded DNA | pH 7.0, 37°C, excision of a single uracil residue from U-G, recombinant enzyme | Monkeypox virus | |
0.967 | - |
uracil-mismatched double-stranded DNA | pH 7.5, 37°C, excision of a single uracil residue from U-G, recombinant enzyme | Monkeypox virus | |
1.83 | - |
uracil-mismatched double-stranded DNA | pH 7.5, 37°C, excision of a single uracil residue from U-T, recombinant enzyme | Monkeypox virus | |
2.18 | - |
uracil-mismatched double-stranded DNA | pH 7.5, 37°C, excision of a single uracil residue from PS-U, recombinant enzyme | Monkeypox virus | |
2.38 | - |
uracil-mismatched double-stranded DNA | pH 7.5, 37°C, excision of a single uracil residue from U-C, recombinant enzyme | Monkeypox virus | |
4.67 | - |
uracil-mismatched double-stranded DNA | pH 7.0, 37°C, excision of a single uracil residue from PS-G, recombinant enzyme | Monkeypox virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Monkeypox virus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH profile | Monkeypox virus |