Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.2.23 extracted from

  • Endutkin, A.V.; Koptelov, S.S.; Popov, A.V.; Torgasheva, N.A.; Lomzov, A.A.; Tsygankova, A.R.; Skiba, T.V.; Afonnikov, D.A.; Zharkov, D.O.
    Residue coevolution reveals functionally important intramolecular interactions in formamidopyrimidine-DNA glycosylase (2018), DNA Repair, 69, 24-33 .
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
E131R loss of activity Escherichia coli
Q234R mutant retains activity Escherichia coli
Q234R/R244E mutant retains activity Escherichia coli
R244E mutant retains activity Escherichia coli
R54E loss of activity Escherichia coli
R54E/E131R loss of activity Escherichia coli
S208A mutation has no effect, in both wild-type and S208 A residue Tyr170 quickly reorients to form an alternative set of hydrogen bonds Escherichia coli
Y170F mutation decreases Fpg binding but does not fully inactivate the protein Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0000039
-
13mer oligonucleotide duplex containing 8-oxoguanine mutant R244E, pH 7.5, 37°C Escherichia coli
0.0000047
-
13mer oligonucleotide duplex containing 8-oxoguanine mutant S208A, pH 7.5, 37°C Escherichia coli
0.000006
-
13mer oligonucleotide duplex containing 8-oxoguanine mutant Q234R, pH 7.5, 37°C Escherichia coli
0.0000081
-
13mer oligonucleotide duplex containing 8-oxoguanine wild-type, pH 7.5, 37°C Escherichia coli
0.0000088
-
13mer oligonucleotide duplex containing 8-oxoguanine mutant Q234R/R244E, pH 7.5, 37°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P05523
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
13mer oligonucleotide duplex containing 8-oxoguanine + H2O
-
Escherichia coli 8-oxoguanine + oligonucleotide
-
?

Synonyms

Synonyms Comment Organism
MutM
-
Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
39
-
mutant Q234R, melting temperature Escherichia coli
39
-
mutant R244E, melting temperature Escherichia coli
44.9
-
mutant Y170F, melting temperature Escherichia coli
46.5
-
mutant Q234R/R244E, melting temperature Escherichia coli
51.2
-
wild-type, melting temperature Escherichia coli
53.8
-
mutant E131R, melting temperature Escherichia coli
54
-
mutant R54E, melting temperature Escherichia coli
54
-
mutant R54E/E131R, melting temperature Escherichia coli
58.2
-
mutant S208A, melting temperature Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0065
-
13mer oligonucleotide duplex containing 8-oxoguanine mutant R244E, pH 7.5, 37°C Escherichia coli
0.0133
-
13mer oligonucleotide duplex containing 8-oxoguanine mutant S208A, pH 7.5, 37°C Escherichia coli
0.0148
-
13mer oligonucleotide duplex containing 8-oxoguanine mutant Q234R/R244E, pH 7.5, 37°C Escherichia coli
0.0153
-
13mer oligonucleotide duplex containing 8-oxoguanine mutant Q234R, pH 7.5, 37°C Escherichia coli
0.0283
-
13mer oligonucleotide duplex containing 8-oxoguanine wild-type, pH 7.5, 37°C Escherichia coli

General Information

General Information Comment Organism
physiological function protein displays three conserved polar bridges (Arg54-Glu131, Gln234-Arg244, and Tyr170-Ser208) located in functional regions of the protein. The coevolving pairs do not form isolated bridges but rather participate in tight local clusters of hydrogen bonds. The Arg54-Glu131 bridge, connecting the N- and C-terminal domains, is important for DNA binding. Mutations of the Gln234-Arg244 bridge, located at the base of the single Fpg beta-hairpin zinc finger, do not affect the activity but sharply decreases the melting temperature of the protein, with the bridge reversal partially restoring the thermal stability Escherichia coli