Protein Variants | Comment | Organism |
---|---|---|
E131R | loss of activity | Escherichia coli |
Q234R | mutant retains activity | Escherichia coli |
Q234R/R244E | mutant retains activity | Escherichia coli |
R244E | mutant retains activity | Escherichia coli |
R54E | loss of activity | Escherichia coli |
R54E/E131R | loss of activity | Escherichia coli |
S208A | mutation has no effect, in both wild-type and S208 A residue Tyr170 quickly reorients to form an alternative set of hydrogen bonds | Escherichia coli |
Y170F | mutation decreases Fpg binding but does not fully inactivate the protein | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000039 | - |
13mer oligonucleotide duplex containing 8-oxoguanine | mutant R244E, pH 7.5, 37°C | Escherichia coli | |
0.0000047 | - |
13mer oligonucleotide duplex containing 8-oxoguanine | mutant S208A, pH 7.5, 37°C | Escherichia coli | |
0.000006 | - |
13mer oligonucleotide duplex containing 8-oxoguanine | mutant Q234R, pH 7.5, 37°C | Escherichia coli | |
0.0000081 | - |
13mer oligonucleotide duplex containing 8-oxoguanine | wild-type, pH 7.5, 37°C | Escherichia coli | |
0.0000088 | - |
13mer oligonucleotide duplex containing 8-oxoguanine | mutant Q234R/R244E, pH 7.5, 37°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P05523 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
13mer oligonucleotide duplex containing 8-oxoguanine + H2O | - |
Escherichia coli | 8-oxoguanine + oligonucleotide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MutM | - |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
39 | - |
mutant Q234R, melting temperature | Escherichia coli |
39 | - |
mutant R244E, melting temperature | Escherichia coli |
44.9 | - |
mutant Y170F, melting temperature | Escherichia coli |
46.5 | - |
mutant Q234R/R244E, melting temperature | Escherichia coli |
51.2 | - |
wild-type, melting temperature | Escherichia coli |
53.8 | - |
mutant E131R, melting temperature | Escherichia coli |
54 | - |
mutant R54E, melting temperature | Escherichia coli |
54 | - |
mutant R54E/E131R, melting temperature | Escherichia coli |
58.2 | - |
mutant S208A, melting temperature | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0065 | - |
13mer oligonucleotide duplex containing 8-oxoguanine | mutant R244E, pH 7.5, 37°C | Escherichia coli | |
0.0133 | - |
13mer oligonucleotide duplex containing 8-oxoguanine | mutant S208A, pH 7.5, 37°C | Escherichia coli | |
0.0148 | - |
13mer oligonucleotide duplex containing 8-oxoguanine | mutant Q234R/R244E, pH 7.5, 37°C | Escherichia coli | |
0.0153 | - |
13mer oligonucleotide duplex containing 8-oxoguanine | mutant Q234R, pH 7.5, 37°C | Escherichia coli | |
0.0283 | - |
13mer oligonucleotide duplex containing 8-oxoguanine | wild-type, pH 7.5, 37°C | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | protein displays three conserved polar bridges (Arg54-Glu131, Gln234-Arg244, and Tyr170-Ser208) located in functional regions of the protein. The coevolving pairs do not form isolated bridges but rather participate in tight local clusters of hydrogen bonds. The Arg54-Glu131 bridge, connecting the N- and C-terminal domains, is important for DNA binding. Mutations of the Gln234-Arg244 bridge, located at the base of the single Fpg beta-hairpin zinc finger, do not affect the activity but sharply decreases the melting temperature of the protein, with the bridge reversal partially restoring the thermal stability | Escherichia coli |