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Literature summary for 3.2.2.23 extracted from

  • Sidorkina, O.M.; Laval, J.
    Role of lysine-57 in the catalytic activities of Escherichia coli formamidopyrimidine-DNA glycosylase (Fpg protein) (1998), Nucleic Acids Res., 26, 5351-5357.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
K155A mutant with reduced 8-oxoguanine-DNA but unchanged Fapy-DNA glycosylase activity Escherichia coli
K57G mutant has dramatically reduced 7,8-dihydro-8-oxoguanine-DNA glycosylase activity and is poorly effective in formation of Schiff base complex with 8-oxoG/C, little effect on 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine-DNA glycosylase activity, no effect on DNA nicking activity at abasic sites Escherichia coli
K57R slight effect of mutation on 7,8-dihydro-8-oxoguanine-DNA glycosylase activity, no effect on 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine-DNA glycosylase activity and on DNA nicking activity at abasic sites Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000004
-
DNA containing 8-hydroxyguanine residues pH 7.8, 37°C, 34-oligomer, wild-type and K57R mutant Fpg Escherichia coli
0.000008
-
DNA containing 8-hydroxyguanine residues pH 7.8, 37°C, 34-oligomer, K57G mutant Fpg Escherichia coli
0.000009 0.000011 DNA containing ring-opened N7-methylguanine residues pH 7.8, 37°C, wild-type, K57G and K57R mutant Fpg Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ possesses a zinc finger Escherichia coli
Zn2+ contains one zinc per enzyme molecule Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
x * 31000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Escherichia coli removes oxidized purines from oxidatively damaged DNA ?
-
?
DNA + H2O Escherichia coli BH20 removes oxidized purines from oxidatively damaged DNA ?
-
?
DNA containing 8-hydroxyguanine residues + H2O Escherichia coli dublex, primary physiological substrate is 7,8-dihydro-8-oxoguanine-DNA, DNA repair 8-hydroxyguanine + DNA
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
containing pFPG220
-
Escherichia coli BH20
-
containing pFPG220
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and K57G, K57R mutant Fpg Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
DNA containing ring-opened N7-methylguanine + H2O = deguanylated DNA + 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine mechanism Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O removes oxidized purines from oxidatively damaged DNA Escherichia coli ?
-
?
DNA + H2O enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity Escherichia coli ?
-
?
DNA + H2O removes oxidized purines from oxidatively damaged DNA Escherichia coli BH20 ?
-
?
DNA + H2O enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity Escherichia coli BH20 ?
-
?
DNA containing 8-hydroxyguanine residues + H2O DNA glycosylase/AP lyase activity Escherichia coli 8-hydroxyguanine + DNA
-
?
DNA containing 8-hydroxyguanine residues + H2O 34mer oligonucleotide containing a single 7,8-dihydro-8-oxoguanine residue Escherichia coli 8-hydroxyguanine + DNA
-
?
DNA containing 8-hydroxyguanine residues + H2O 7,8-dihydro-8-oxoguanine opposite C dublex DNA, formation of a Schiff base intermediate, important role for Lys-57 in the 7,8-dihydro-8-oxoG-DNA glycolase activity in vitro and in vivo Escherichia coli 8-hydroxyguanine + DNA
-
?
DNA containing 8-hydroxyguanine residues + H2O 7,8-dihydro-8-oxoguanine-DNA Escherichia coli 8-hydroxyguanine + DNA
-
?
DNA containing 8-hydroxyguanine residues + H2O dublex, primary physiological substrate is 7,8-dihydro-8-oxoguanine-DNA, DNA repair Escherichia coli 8-hydroxyguanine + DNA
-
?
DNA containing 8-hydroxyguanine residues + H2O DNA glycosylase/AP lyase activity Escherichia coli BH20 8-hydroxyguanine + DNA
-
?
DNA containing 8-hydroxyguanine residues + H2O 34mer oligonucleotide containing a single 7,8-dihydro-8-oxoguanine residue Escherichia coli BH20 8-hydroxyguanine + DNA
-
?
DNA containing 8-hyroxyguanine + H2O
-
Escherichia coli ?
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
-
Escherichia coli 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
-
Escherichia coli BH20 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
?

Subunits

Subunits Comment Organism
? x * 31000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000167
-
DNA containing 8-hydroxyguanine residues pH 7.8, 37°C, 34-oligomer, K57G mutant Fpg Escherichia coli
0.00167
-
DNA containing 8-hyroxyguanine pH 7.8, 37°C, 34-oligomer, K57R mutant Fpg Escherichia coli
0.002
-
DNA containing ring-opened N7-methylguanine residues pH 7.8, 37°C, K57G and K57R mutant Fpg Escherichia coli
0.00717
-
DNA containing 8-hydroxyguanine residues pH 7.8, 37°C, 34-oligomer, wild-type Fpg Escherichia coli
0.00833
-
DNA containing ring-opened N7-methylguanine residues pH 7.8, 37°C, wild-type Fpg Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Escherichia coli