Protein Variants | Comment | Organism |
---|---|---|
K155A | mutant with reduced 8-oxoguanine-DNA but unchanged Fapy-DNA glycosylase activity | Escherichia coli |
K57G | mutant has dramatically reduced 7,8-dihydro-8-oxoguanine-DNA glycosylase activity and is poorly effective in formation of Schiff base complex with 8-oxoG/C, little effect on 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine-DNA glycosylase activity, no effect on DNA nicking activity at abasic sites | Escherichia coli |
K57R | slight effect of mutation on 7,8-dihydro-8-oxoguanine-DNA glycosylase activity, no effect on 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine-DNA glycosylase activity and on DNA nicking activity at abasic sites | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000004 | - |
DNA containing 8-hydroxyguanine residues | pH 7.8, 37°C, 34-oligomer, wild-type and K57R mutant Fpg | Escherichia coli | |
0.000008 | - |
DNA containing 8-hydroxyguanine residues | pH 7.8, 37°C, 34-oligomer, K57G mutant Fpg | Escherichia coli | |
0.000009 | 0.000011 | DNA containing ring-opened N7-methylguanine residues | pH 7.8, 37°C, wild-type, K57G and K57R mutant Fpg | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | possesses a zinc finger | Escherichia coli | |
Zn2+ | contains one zinc per enzyme molecule | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
x * 31000, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | Escherichia coli | removes oxidized purines from oxidatively damaged DNA | ? | - |
? | |
DNA + H2O | Escherichia coli BH20 | removes oxidized purines from oxidatively damaged DNA | ? | - |
? | |
DNA containing 8-hydroxyguanine residues + H2O | Escherichia coli | dublex, primary physiological substrate is 7,8-dihydro-8-oxoguanine-DNA, DNA repair | 8-hydroxyguanine + DNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
containing pFPG220 | - |
Escherichia coli BH20 | - |
containing pFPG220 | - |
Purification (Comment) | Organism |
---|---|
wild-type and K57G, K57R mutant Fpg | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
DNA containing ring-opened N7-methylguanine + H2O = deguanylated DNA + 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine | mechanism | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | removes oxidized purines from oxidatively damaged DNA | Escherichia coli | ? | - |
? | |
DNA + H2O | enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity | Escherichia coli | ? | - |
? | |
DNA + H2O | removes oxidized purines from oxidatively damaged DNA | Escherichia coli BH20 | ? | - |
? | |
DNA + H2O | enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity | Escherichia coli BH20 | ? | - |
? | |
DNA containing 8-hydroxyguanine residues + H2O | DNA glycosylase/AP lyase activity | Escherichia coli | 8-hydroxyguanine + DNA | - |
? | |
DNA containing 8-hydroxyguanine residues + H2O | 34mer oligonucleotide containing a single 7,8-dihydro-8-oxoguanine residue | Escherichia coli | 8-hydroxyguanine + DNA | - |
? | |
DNA containing 8-hydroxyguanine residues + H2O | 7,8-dihydro-8-oxoguanine opposite C dublex DNA, formation of a Schiff base intermediate, important role for Lys-57 in the 7,8-dihydro-8-oxoG-DNA glycolase activity in vitro and in vivo | Escherichia coli | 8-hydroxyguanine + DNA | - |
? | |
DNA containing 8-hydroxyguanine residues + H2O | 7,8-dihydro-8-oxoguanine-DNA | Escherichia coli | 8-hydroxyguanine + DNA | - |
? | |
DNA containing 8-hydroxyguanine residues + H2O | dublex, primary physiological substrate is 7,8-dihydro-8-oxoguanine-DNA, DNA repair | Escherichia coli | 8-hydroxyguanine + DNA | - |
? | |
DNA containing 8-hydroxyguanine residues + H2O | DNA glycosylase/AP lyase activity | Escherichia coli BH20 | 8-hydroxyguanine + DNA | - |
? | |
DNA containing 8-hydroxyguanine residues + H2O | 34mer oligonucleotide containing a single 7,8-dihydro-8-oxoguanine residue | Escherichia coli BH20 | 8-hydroxyguanine + DNA | - |
? | |
DNA containing 8-hyroxyguanine + H2O | - |
Escherichia coli | ? | - |
? | |
DNA containing ring-opened N7-methylguanine residues + H2O | - |
Escherichia coli | 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA | - |
? | |
DNA containing ring-opened N7-methylguanine residues + H2O | - |
Escherichia coli BH20 | 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000167 | - |
DNA containing 8-hydroxyguanine residues | pH 7.8, 37°C, 34-oligomer, K57G mutant Fpg | Escherichia coli | |
0.00167 | - |
DNA containing 8-hyroxyguanine | pH 7.8, 37°C, 34-oligomer, K57R mutant Fpg | Escherichia coli | |
0.002 | - |
DNA containing ring-opened N7-methylguanine residues | pH 7.8, 37°C, K57G and K57R mutant Fpg | Escherichia coli | |
0.00717 | - |
DNA containing 8-hydroxyguanine residues | pH 7.8, 37°C, 34-oligomer, wild-type Fpg | Escherichia coli | |
0.00833 | - |
DNA containing ring-opened N7-methylguanine residues | pH 7.8, 37°C, wild-type Fpg | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Escherichia coli |