Cloned (Comment) | Organism |
---|---|
isolation of 2 cDNA clones: alternative splicing products AtFPG-1 and -2 of a single gene with different C-terminal sequences, amino acid sequences, expression of both proteins in Escherichia coli BL21(DE3) | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no zinc finger | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32400 | - |
x * 32400, AtFPG-2, calculated from the amino acid sequence | Arabidopsis thaliana |
34000 | - |
x * 34000, AtFPG-2, SDS-PAGE | Arabidopsis thaliana |
44800 | - |
x * 44800, AtFPG-1, calculated from the amino acid sequence | Arabidopsis thaliana |
46000 | - |
x * 46000, AtFPG-1, SDS-PAGE | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | Arabidopsis thaliana | catalyzes the initial steps in the repair of DNA containing oxidized purines | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O80358 | AtFPG-1, enzyme exists in 2 splicing variants: AtFPG-1 and -2 | - |
Arabidopsis thaliana | O80358 | AtFPG-2, enzyme exists in 2 splicing variants: AtFPG-1 and -2 | - |
Purification (Comment) | Organism |
---|---|
recombinant AtFPG-1, expressed in Escherichia coli BL21(DE3) | Arabidopsis thaliana |
recombinant AtFPG-2, expressed in Escherichia coli BL21(DE3) | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | both AtFPG-1 and -2 cleave DNA containing apurinic sites and UV-irradiated and oxidized DNA | Arabidopsis thaliana | ? | - |
? | |
DNA + H2O | catalyzes the initial steps in the repair of DNA containing oxidized purines | Arabidopsis thaliana | ? | - |
? | |
DNA containing 8-hydroxyguanine residues + H2O | AtFPG-1, but not AtFPG-2, cleaves double-stranded oligonucleotides containing 8-oxoguanine | Arabidopsis thaliana | 8-hydroxyguanine + DNA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32400, AtFPG-2, calculated from the amino acid sequence | Arabidopsis thaliana |
? | x * 44800, AtFPG-1, calculated from the amino acid sequence | Arabidopsis thaliana |
? | x * 46000, AtFPG-1, SDS-PAGE | Arabidopsis thaliana |
? | x * 34000, AtFPG-2, SDS-PAGE | Arabidopsis thaliana |