Protein Variants | Comment | Organism |
---|---|---|
K155A | effect of mutation on specificity, mutant with very low activity, kinetic study | Escherichia coli |
K57G | effect of mutation on specificity, mutant removes FapyAde and FapyGua with reduced activity compared to wild-type Fpg, kinetic study | Escherichia coli |
K57R | effect of mutation on specificity, mutant removes FapyAde and FapyGua with reduced activity compared to wild-type Fpg, kinetic study | Escherichia coli |
P2E | effect of mutation on specificity, inactive mutant, kinetic study | Escherichia coli |
P2G | effect of mutation on specificity, mutant with very low activity, kinetic study | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00078 | 0.00084 | DNA containing 4,6-diamino-5-formamidopyrimidine residues | pH 7.4, 37°C, K57G mutant Fpg | Escherichia coli | |
0.00079 | 0.0009 | DNA containing 4,6-diamino-5-formamidopyrimidine residues | pH 7.4, 37°C, K57R mutant Fpg | Escherichia coli | |
0.00093 | 0.00155 | DNA containing 8-hydroxyguanine residues | pH 7.4, 37°C, K57R mutant Fpg | Escherichia coli | |
0.00107 | 0.00129 | DNA containing 4,6-diamino-5-formamidopyrimidine residues | pH 7.4, 37°C, wild-type Fpg | Escherichia coli | |
0.00109 | - |
DNA containing 8-hydroxyguanine residues | pH 7.4, 37°C, wild-type Fpg, from DNA treated with H2O2/Fe(III)-EDTA/asc | Escherichia coli | |
0.00126 | - |
DNA containing 8-hydroxyguanine residues | pH 7.4, 37°C, K57G mutant Fpg | Escherichia coli | |
0.00178 | - |
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues | pH 7.4, 37°C, K57R mutant Fpg, from DNA gamma-irradiated under NO2 | Escherichia coli | |
0.00187 | - |
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues | pH 7.4, 37°C, K57G mutant Fpg, from DNA gamma-irradiated under NO2 | Escherichia coli | |
0.00311 | - |
DNA containing 8-hydroxyguanine residues | pH 7.4, 37°C, wild-type Fpg, from DNA gamma-irradiated under NO2 | Escherichia coli | |
0.00343 | 0.00376 | DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues | pH 7.4, 37°C, wild-type Fpg | Escherichia coli | |
0.00422 | - |
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues | pH 7.4, 37°C, K57G mutant Fpg, from DNA treated with H2O2/Fe(III)-EDTA/asc | Escherichia coli | |
0.00487 | - |
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues | pH 7.4, 37°C, K57R mutant Fpg, from DNA treated with H2O2/Fe(III)-EDTA/asc | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | contains a four cystein-zinc finger motif | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | Escherichia coli | DNA repair enzyme specific for the removal of purine-derived lesions from DNA damaged by free radicals and other oxidative processes | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
K57R, K155A mutant Fpg | Escherichia coli |
wild-type and K57G, P2G, P2E mutant Fpg | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | removes oxidized purines from oxidatively damaged DNA | Escherichia coli | ? | - |
? | |
DNA + H2O | active site is located within the first 73 amino acids of the N-terminus | Escherichia coli | ? | - |
? | |
DNA + H2O | enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity | Escherichia coli | ? | - |
? | |
DNA + H2O | DNA repair enzyme specific for the removal of purine-derived lesions from DNA damaged by free radicals and other oxidative processes | Escherichia coli | ? | - |
? | |
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O | removal with similar specificity as 4,6-diamino-5-formamidopyrimidine and 8-hydroxyguanine | Escherichia coli | 2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA | - |
? | |
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O | FapyGua | Escherichia coli | 2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA | - |
? | |
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O | removal with similar specificity as 2,6-diamino-4-hydroxy-5-formamidopyrimidine and 8-hydroxyguanine | Escherichia coli | 4,6-diamino-5-formamidopyrimidine + DNA | - |
? | |
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O | FapyAde | Escherichia coli | 4,6-diamino-5-formamidopyrimidine + DNA | - |
? | |
DNA containing 8-hydroxyadenine residues + H2O | poor substrate | Escherichia coli | 8-hydroxyadenine + DNA | - |
? | |
DNA containing 8-hydroxyguanine residues + H2O | removal with similar specificity as 4,6-diamino-5-formamidopyrimidine and 2,6-diamino-4-hydroxy-5-formamidopyrimidine, important role of Lys-57 in Fpg activity for 8-hydroxyguanine, but lesser significant role for formamidopyrimidines, Pro-2 is critical for substrate recognition and in catalysis of its excision | Escherichia coli | 8-hydroxyguanine + DNA | - |
? | |
DNA containing ring-opened N7-methylguanine residues + H2O | - |
Escherichia coli | 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Escherichia coli |