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Literature summary for 3.2.2.22 extracted from

  • Au, K.Y.; Wang, R.R.; Wong, Y.T.; Wong, K.B.; Zheng, Y.T.; Shaw, P.C.
    Engineering a switch-on peptide to ricin A chain for increasing its specificity towards HIV-infected cells (2014), Biochim. Biophys. Acta, 1840, 958-963.
    View publication on PubMed
Search for more literature for 3.2.2.22

Application

Application Comment Organism
medicine C-terminal enzymatically active ricin A chain variants are specifically cleaved by HIV-1 protease both in vitro and in HIV-infected cells and the products mediate specific inhibitory effect towards HIV replication. Upon proteolysis, the processed variants show enhanced antiviral effect with low cytotoxicity towards uninfected cells Ricinus communis

Cloned(Commentary)

Cloned (Comment) Organism
the enzymatically active ricin A chain is expressed in Escherichia coli BL21(DE3)pLysS cells Ricinus communis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
rRNA + H2O Ricinus communis
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Ricinus communis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex 200 gel filtration Ricinus communis

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Ricinus communis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
rRNA + H2O
-
 Ricinus communis ?
-
 ?

Synonyms

Synonyms Comment Organism
ribosome-inactivating protein
-
 Ricinus communis
ricin
-
 Ricinus communis
RIP
-
 Ricinus communis
RTA enzymatically active ricin A chain Ricinus communis