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Literature summary for 3.2.2.22 extracted from

  • Too, P.H.; Ma, M.K.; Mak, A.N.; Wong, Y.T.; Tung, C.K.; Zhu, G.; Au, S.W.; Wong, K.B.; Shaw, P.C.
    The C-terminal fragment of the ribosomal P protein complexed to trichosanthin reveals the interaction between the ribosome-inactivating protein and the ribosome (2009), Nucleic Acids Res., 37, 602-610.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.22

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) pLysS Trichosanthes kirilowii

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with 11-mer of P-protein C-terminal end (c11-P) Trichosanthes kirilowii

Protein Variants

Protein Variants Comment Organism
K173A/R174A/K177A no ribosome interaction, drastically reduced N-glycosidase acitivity Trichosanthes kirilowii
V232K/N236D 17-fold reduced activity compared to wild-type, no ribosome interaction, V232K is at mouth of hydrophobic pocket, where the LF motif of c11-P binds, expected to block the access of the P-protein to the pocket, N236D substitution removes hydrogen bond between the amide of Asn236 and the backbone carbonyl of Leu9 of c11-P Trichosanthes kirilowii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Trichosanthes kirilowii depurination of specific adenine at sarcin-ricin loop of 28S rRNA, preventing the binding of elongation factors to the GTPase activation centre of the ribosome ?
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 ?

Organism

Organism UniProt Comment Textmining
Trichosanthes kirilowii P09989 medicinal plant with anti-tumor and anti-HIV properties
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Purification (Commentary)

Purification (Comment) Organism
Hi-Trap CM-Sepharose column followed by Hi-Trap SP-Sepharose column with 20 mM phosphate buffer, pH 6.7 Trichosanthes kirilowii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information depurination of specific adenine at sarcin-ricin loop of 28S rRNA, preventing the binding of elongation factors to the GTPase activation centre of the ribosome Trichosanthes kirilowii ?
-
 ?
rRNA + H2O depurination of A-4324 of 28S rRNA, inhibition of protein synthesis in a nuclease-untreated rabbit reticulocyte lysate Trichosanthes kirilowii ?
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 ?

Synonyms

Synonyms Comment Organism
trichosanthin TCS Trichosanthes kirilowii