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Literature summary for 3.2.2.22 extracted from

  • Roday, S.; Sturm, M.B.; Blakaj, D.; Schramm, V.L.
    Detection of an abasic site in RNA with stem-loop DNA beacons: application to an activity assay for Ricin Toxin A-Chain (2008), J. Biochem. Biophys. Methods, 70, 945-953.
    View publication on PubMed

Application

Application Comment Organism
diagnostics identification of substrate and product of ricin toxin A-chain with 3 different DNA stem-loop probes with fluorophore and quencher linked to the 5'- and 3'-ends or vice versa hybridized to substrate or ricin, respectively, lower limit of detection 14 ng/ml of ricin toxin chain a Ricinus communis

Organism

Organism UniProt Comment Textmining
Ricinus communis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
14-mer rRNA with GAGA tetraloop + H2O substrate and product with specific DNA loop probe, fluorophore and quencher, 37°C, citrate buffer, pH 4.0, than neutralization to pH 7.6 and hybridization with DNA-probes Ricinus communis 14-mer rRNA with GabGA + ?
-
?

Synonyms

Synonyms Comment Organism
ricin toxin A-chain
-
Ricinus communis
RTA
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Ricinus communis