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Literature summary for 3.2.2.22 extracted from

  • Baldwin, A.E.; Khan, M.A.; Tumer, N.E.; Goss, D.J.; Friedland, D.E.
    Characterization of pokeweed antiviral protein binding to mRNA cap analogs: competition with nucleotides and enhancement by translation initiation factor iso4G (2009), Biochim. Biophys. Acta, 1789, 109-116.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Phytolacca americana removal of adenine from rRNA, inactivating ribosomal function in translation ?
-
?

Organism

Organism UniProt Comment Textmining
Phytolacca americana
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pokeweed
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Purification (Commentary)

Purification (Comment) Organism
homogenized frozen leaves filtered through cheesecloth, centrifuged, saturated with ammonium sulfate to 60%, stirred, all at 4 degrees Celsius, centrifuged, pellet resuspended in 20 mM Tris-HCl, pH 7.5 with 1 mM beta-mercaptoethanol, and 0.2 mM EDTA, dialyzed against buffer, centrifuged, loaded onto HiTrap Q FF column, bound proteins loaded onto HiTrap SP FF column, eluted with NaCl gradient, PAP elutes at about 250 mM NaCl Phytolacca americana

Source Tissue

Source Tissue Comment Organism Textmining
leaf
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Phytolacca americana
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-anthraniloyl 7-methylguanosine triphosphate + H2O depurination downstream of cap structure, 2.4-fold enhanced binding to m7GTP upon the addition of translation initiation factor G4 (elFiso4G) Phytolacca americana ?
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?
additional information removal of adenine from rRNA, inactivating ribosomal function in translation Phytolacca americana ?
-
?

Synonyms

Synonyms Comment Organism
PAP
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Phytolacca americana
pokeweed antiviral protein
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Phytolacca americana
ribosome inactivating protein
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Phytolacca americana

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 32
-
Phytolacca americana