Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Phytolacca americana | removal of adenine from rRNA, inactivating ribosomal function in translation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phytolacca americana | - |
pokeweed | - |
Purification (Comment) | Organism |
---|---|
homogenized frozen leaves filtered through cheesecloth, centrifuged, saturated with ammonium sulfate to 60%, stirred, all at 4 degrees Celsius, centrifuged, pellet resuspended in 20 mM Tris-HCl, pH 7.5 with 1 mM beta-mercaptoethanol, and 0.2 mM EDTA, dialyzed against buffer, centrifuged, loaded onto HiTrap Q FF column, bound proteins loaded onto HiTrap SP FF column, eluted with NaCl gradient, PAP elutes at about 250 mM NaCl | Phytolacca americana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Phytolacca americana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-anthraniloyl 7-methylguanosine triphosphate + H2O | depurination downstream of cap structure, 2.4-fold enhanced binding to m7GTP upon the addition of translation initiation factor G4 (elFiso4G) | Phytolacca americana | ? | - |
? | |
additional information | removal of adenine from rRNA, inactivating ribosomal function in translation | Phytolacca americana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PAP | - |
Phytolacca americana |
pokeweed antiviral protein | - |
Phytolacca americana |
ribosome inactivating protein | - |
Phytolacca americana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 32 | - |
Phytolacca americana |