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Literature summary for 3.2.2.22 extracted from
- Cochinin B, a novel ribosome-inactivating protein from the seeds of Momordica cochinchinensis (2007), Biol. Pharm. Bull., 30, 428-432.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
gel filtration | Momordica cochinchinensis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Momordica cochinchinensis | enzyme shows strong inhibitory activity on protein synthesis in rabbit cell-free reticulocyte lysate system with IC50 value of 0.36nM. Enzyme is cytotoxic. IC50 values of Cochinin B are 16.9, 114 and 574 nM for human cervical epithelial carcinoma cell, HEK 293 and NCI-H187 cell, respectively | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Momordica cochinchinensis | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Momordica cochinchinensis | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, stable for months | Momordica cochinchinensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 28S rRNA + H2O | source: ribosome of HeLa cell | Momordica cochinchinensis | ? | depurination of rRNA and release of RNA fragment of approximately 400 nucleotides | ? | |
| additional information | enzyme shows strong inhibitory activity on protein synthesis in rabbit cell-free reticulocyte lysate system with IC50 value of 0.36nM. Enzyme is cytotoxic. IC50 values of Cochinin B are 16.9, 114 and 574 nM for human cervical epithelial carcinoma cell, HEK 293 and NCI-H187 cell, respectively | Momordica cochinchinensis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 28000, SDS-PAGE | Momordica cochinchinensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cochinin B | - |
Momordica cochinchinensis |
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Release 2023.1 (January 2023)
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