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Literature summary for 3.2.2.22 extracted from
- Intein-mediated fusion expression, high efficient refolding, and one-step purification of gelonin toxin (2004), Protein Expr. Purif., 37, 361-367.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| functional expression of CBD-intein-Gel fusion protein in Escherichia coli strain Bl21(DE3) in inclusion bodies | Suregada multiflora |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of a mutant gelonin fused to a chitin binding domain-intein, i.e. CBD-intein-Gel | Suregada multiflora |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Suregada multiflora | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant refolded CBD-intein-Gel fusion protein from inclusion bodies by chitin affinity chromatography with elution using an intein C-terminal self-cleavage method, 96% purity | Suregada multiflora |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| recombinant CBD-intein-Gel fusion protein expressed in Escherichia coli strain Bl21(DE3) from inclusion bodies by denaturation and stepwise dialysis, 69% of the protein is refolded to the native state in presence of 0.02 mM GSSG and 0.2 mM GSH in 10 mM Tris-HCl, 0.5 M NaCl, pH 8.5, and 0.5 M L-arginine | Suregada multiflora |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the recombinant gelonin fusion protein inhibits protein synthesis in a rabbit reticulocyte lysate | Suregada multiflora | ? | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Suregada multiflora |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| gelonin | - |
Suregada multiflora |
| type I ribosome-inactivating protein | - |
Suregada multiflora |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Suregada multiflora |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Suregada multiflora |
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