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Literature summary for 3.2.2.22 extracted from

  • Vandenbussche, F.; Peumans, W.J.; Desmyter, S.; Proost, P.; Ciani, M.; Van Damme, E.J.
    The type-1 and type-2 ribosome-inactivating proteins from Iris confer transgenic tobacco plants local but not systemic protection against viruses (2004), Planta, 220, 211-221.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of IRAb in transgenic Nicotiana tabacum cv. Samsun plant leaves Iris sp.
expression of IRIP in transgenic Nicotiana tabacum cv. Samsun plants Iris sp.

Protein Variants

Protein Variants Comment Organism
additional information construction of transgenic Nicotiana tabacum plants expressing the type-1 RIP IRIP from Iris sp., the transgenic plants show increased resistance against the tobacco mosaic and tobacco etch viruses compared to wild-type plants, and impaired development of roots as well as phenotypic aberrations, such as stunt growth due to a shortening in internodes, thickened, smaller and narrower leaves and chlorosis at age of seven weeks, overview, the enzyme acts cytotoxic in tobacco cells Iris sp.
additional information construction of transgenic Nicotiana tabacum plants expressing the type-2 RIP IRAb from Iris sp., the transgenic plants show increased resistance against the tobacco mosaic and tobacco etch viruses compared to wild-type plants, and impaired development of roots as well as phenotypic aberrations, such as stunt growth due to a shortening in internodes, thickened, smaller and narrower leaves and chlorosis at age of seven weeks, overview, the enzyme acts cytotoxic in tobacco cells Iris sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Iris sp. IRAb shows strong local antiviral activity against the tobacco mosaic virus, but cannot confersystemic protection against viruses, e.g. the tobacco etch virus ?
-
?
additional information Iris sp. IRIP shows strong local antiviral activity against the tobacco mosaic virus, but cannot confer systemic protection against viruses, e.g. the tobacco etch virus ?
-
?

Organism

Organism UniProt Comment Textmining
Iris sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant IRAb from transgenic Nicotiana tabacum cv. Samsun plant leaves by ammonium sulfate fractionation, galactose affinity chromatography, hydrophobic interaction chromatography, and dialysis Iris sp.

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
tobacco mosaic virus infection inhibition rates in transgenic plants expressing IRAb, overview Iris sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information IRAb shows strong local antiviral activity against the tobacco mosaic virus, but cannot confersystemic protection against viruses, e.g. the tobacco etch virus Iris sp. ?
-
?
additional information IRIP shows strong local antiviral activity against the tobacco mosaic virus, but cannot confer systemic protection against viruses, e.g. the tobacco etch virus Iris sp. ?
-
?
additional information IRAb shows strong local antiviral activity against the tobacco mosaic virus Iris sp. ?
-
?
additional information IRIP shows strong local antiviral activity against the tobacco mosaic virus Iris sp. ?
-
?

Subunits

Subunits Comment Organism
More overall enzyme structure, overview Iris sp.

Synonyms

Synonyms Comment Organism
IRAb
-
Iris sp.
IRIP
-
Iris sp.
Iris agglutinin b
-
Iris sp.
Iris ribosome-inactivating protein
-
Iris sp.
PAG
-
Iris sp.
polynucleotide:adenosine glycosidase
-
Iris sp.
type I ribosome-inactivating protein
-
Iris sp.
type I RIP
-
Iris sp.
type II ribosome-inactivating protein
-
Iris sp.
type II RIP
-
Iris sp.
type-1 ribosome-inactivating protein
-
Iris sp.
type-1 RIP
-
Iris sp.
type-2 ribosome-inactivating protein
-
Iris sp.
type-2 RIP
-
Iris sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Iris sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Iris sp.