Cloned (Comment) | Organism |
---|---|
expression of IRAb in transgenic Nicotiana tabacum cv. Samsun plant leaves | Iris sp. |
expression of IRIP in transgenic Nicotiana tabacum cv. Samsun plants | Iris sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of transgenic Nicotiana tabacum plants expressing the type-1 RIP IRIP from Iris sp., the transgenic plants show increased resistance against the tobacco mosaic and tobacco etch viruses compared to wild-type plants, and impaired development of roots as well as phenotypic aberrations, such as stunt growth due to a shortening in internodes, thickened, smaller and narrower leaves and chlorosis at age of seven weeks, overview, the enzyme acts cytotoxic in tobacco cells | Iris sp. |
additional information | construction of transgenic Nicotiana tabacum plants expressing the type-2 RIP IRAb from Iris sp., the transgenic plants show increased resistance against the tobacco mosaic and tobacco etch viruses compared to wild-type plants, and impaired development of roots as well as phenotypic aberrations, such as stunt growth due to a shortening in internodes, thickened, smaller and narrower leaves and chlorosis at age of seven weeks, overview, the enzyme acts cytotoxic in tobacco cells | Iris sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Iris sp. | IRAb shows strong local antiviral activity against the tobacco mosaic virus, but cannot confersystemic protection against viruses, e.g. the tobacco etch virus | ? | - |
? | |
additional information | Iris sp. | IRIP shows strong local antiviral activity against the tobacco mosaic virus, but cannot confer systemic protection against viruses, e.g. the tobacco etch virus | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Iris sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant IRAb from transgenic Nicotiana tabacum cv. Samsun plant leaves by ammonium sulfate fractionation, galactose affinity chromatography, hydrophobic interaction chromatography, and dialysis | Iris sp. |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
tobacco mosaic virus infection inhibition rates in transgenic plants expressing IRAb, overview | Iris sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | IRAb shows strong local antiviral activity against the tobacco mosaic virus, but cannot confersystemic protection against viruses, e.g. the tobacco etch virus | Iris sp. | ? | - |
? | |
additional information | IRIP shows strong local antiviral activity against the tobacco mosaic virus, but cannot confer systemic protection against viruses, e.g. the tobacco etch virus | Iris sp. | ? | - |
? | |
additional information | IRAb shows strong local antiviral activity against the tobacco mosaic virus | Iris sp. | ? | - |
? | |
additional information | IRIP shows strong local antiviral activity against the tobacco mosaic virus | Iris sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | overall enzyme structure, overview | Iris sp. |
Synonyms | Comment | Organism |
---|---|---|
IRAb | - |
Iris sp. |
IRIP | - |
Iris sp. |
Iris agglutinin b | - |
Iris sp. |
Iris ribosome-inactivating protein | - |
Iris sp. |
PAG | - |
Iris sp. |
polynucleotide:adenosine glycosidase | - |
Iris sp. |
type I ribosome-inactivating protein | - |
Iris sp. |
type I RIP | - |
Iris sp. |
type II ribosome-inactivating protein | - |
Iris sp. |
type II RIP | - |
Iris sp. |
type-1 ribosome-inactivating protein | - |
Iris sp. |
type-1 RIP | - |
Iris sp. |
type-2 ribosome-inactivating protein | - |
Iris sp. |
type-2 RIP | - |
Iris sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Iris sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Iris sp. |