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Literature summary for 3.2.2.22 extracted from

  • Rojo, M.A.; Yato, M.; Ishii-Minami, N.; Minami, E.; Kaku, H.; Citores, L.; Girbes, T.; Shibuya, N.
    Isolation, cDNA cloning, biological properties, and carbohydrate binding specificity of sieboldin-b, a type II ribosome-inactivating protein from the bark of Japanese elderberry (Sambucus sieboldiana) (1997), Arch. Biochem. Biophys., 340, 185-194.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cDNA cloning Sambucus racemosa subsp. sieboldiana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
1 * 27000 + 1 * 33000, SDS-PAGE Sambucus racemosa subsp. sieboldiana
33000
-
1 * 27000 + 1 * 33000, SDS-PAGE Sambucus racemosa subsp. sieboldiana
59400
-
time-of-flight mass spectrometry Sambucus racemosa subsp. sieboldiana
61000
-
gel filtration Sambucus racemosa subsp. sieboldiana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
rRNA + H2O Sambucus racemosa subsp. sieboldiana
-
?
-
?

Organism

Organism UniProt Comment Textmining
Sambucus racemosa subsp. sieboldiana
-
japanese elderberry
-

Purification (Commentary)

Purification (Comment) Organism
-
Sambucus racemosa subsp. sieboldiana

Source Tissue

Source Tissue Comment Organism Textmining
bark
-
Sambucus racemosa subsp. sieboldiana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
specific activity 0.00111 U mg-1, 1 unit is the amount of enzyme required for 50% inhibition of protein synthesis in 1 ml Sambucus racemosa subsp. sieboldiana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
rRNA + H2O
-
Sambucus racemosa subsp. sieboldiana ?
-
?

Subunits

Subunits Comment Organism
dimer 1 * 27000 + 1 * 33000, SDS-PAGE Sambucus racemosa subsp. sieboldiana

Synonyms

Synonyms Comment Organism
sieboldin-b
-
Sambucus racemosa subsp. sieboldiana