Cloned (Comment) | Organism |
---|---|
synthetic gene variants expressed in Escherichia coli MM294 | Mirabilis jalapa |
Protein Variants | Comment | Organism |
---|---|---|
E168D | replacing DNA fragments of the total synthetic gene of MAP, weaker inhibitory effect | Mirabilis jalapa |
R26L | replacing DNA fragments of the total synthetic gene of MAP | Mirabilis jalapa |
R48L | replacing DNA fragments of the total synthetic gene of MAP | Mirabilis jalapa |
Y118F | replacing DNA fragments of the total synthetic gene of MAP, slightly stronger inhibitory effect | Mirabilis jalapa |
Y72F | replacing DNA fragments of the total synthetic gene of MAP, slightly stronger inhibitory effect | Mirabilis jalapa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
rRNA + H2O | Mirabilis jalapa | inhibits mechanical transmission of plant viruses such as tobacco mosaic virus | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mirabilis jalapa | - |
Mirabilis antiviral protein, MAP, MAP variants can maintain their N-glycosidase activity when the conserved Glu-168 and Arg-171 are changed to similar charged residues | - |
Purification (Comment) | Organism |
---|---|
wild-type and recombinant proteins | Mirabilis jalapa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Mirabilis jalapa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | inactivates both eukaryotic and procaryotic ribosomes by means of site-specific RNA N-glycosidase activity, inhibits mechanical transmission of plant viruses | Mirabilis jalapa | ? | - |
? | |
rRNA + H2O | - |
Mirabilis jalapa | ? | - |
? | |
rRNA + H2O | inhibits mechanical transmission of plant viruses such as tobacco mosaic virus | Mirabilis jalapa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MAP | MAP, a MAP variant with Arg-26 changed to Leu is designated R26L, a MAP variant with Arg-28 changed to Leu is designated R28L | Mirabilis jalapa |