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Literature summary for 3.2.2.21 extracted from

  • Admiraal, S.; O'Brien, P.
    DNA-N-glycosylases process novel O-glycosidic sites in DNA (2013), Biochemistry, 52, 4066-4074.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.21

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
D238N inactive Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alkylated DNA + H2O Escherichia coli
-
 3-methyladenine + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P04395
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Source S cation exchange column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-propanediol-DNA + H2O
-
 Escherichia coli ?
-
 ?
1,3-propanediol-DNA + H2O
-
 Escherichia coli ?
-
 ?
alkylated DNA + H2O
-
 Escherichia coli 3-methyladenine + ?
-
 ?
ethanol-DNA + H2O best substrate Escherichia coli ?
-
 ?
ethylene glycol-DNA + H2O
-
 Escherichia coli ?
-
 ?
glycerol-DNA + H2O
-
 Escherichia coli ?
-
 ?
methanol-DNA + H2O
-
 Escherichia coli ?
-
 ?
additional information the enzyme is able to hydrolyze O-glycosidic bonds in addition to N-glycosyl bonds Escherichia coli ?
-
 ?
propanol-DNA + H2O
-
 Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
3-methyladenine DANN glycosylase II
-
 Escherichia coli
AlkA
-
 Escherichia coli