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Literature summary for 3.2.2.21 extracted from

  • Masaoka, A.; Terato, H.; Kobayashi, M.; Honsho, A.; Ohyama, Y.; Ide, H.
    Enzymatic repair of 5-formyluracil. I. Excision of 5-formyluracil site-specifically incorporated into oligonucleotide substrates by alka protein (Escherichia coli 3-methyladenine DNA glycosylase II) (1999), J. Biol. Chem., 274, 25136-25143.
    View publication on PubMed
Search for more literature for 3.2.2.21

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000021
-
 alkylated DNA pH 8.0, 37°C, excision of 5-formyluracil Escherichia coli
0.00003
-
 alkylated DNA pH 8.0, 37°C, excision of 7-methylguanine Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alkylated DNA + H2O Escherichia coli inducible pathway for repair of DNA damaged by simple alkylating agents such as methylmethanesulfonate and N-methyl-N'-nitro-N-nitrosoguanidine 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA
-
 ?
alkylated DNA + H2O Escherichia coli MV1161 inducible pathway for repair of DNA damaged by simple alkylating agents such as methylmethanesulfonate and N-methyl-N'-nitro-N-nitrosoguanidine 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 MV1161
-
Escherichia coli MV1161
-
 MV1161
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alkylated DNA + H2O
-
 Escherichia coli 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA
-
 ?
alkylated DNA + H2O inducible pathway for repair of DNA damaged by simple alkylating agents such as methylmethanesulfonate and N-methyl-N'-nitro-N-nitrosoguanidine Escherichia coli 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA
-
 ?
alkylated DNA + H2O
-
 Escherichia coli MV1161 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA
-
 ?
alkylated DNA + H2O inducible pathway for repair of DNA damaged by simple alkylating agents such as methylmethanesulfonate and N-methyl-N'-nitro-N-nitrosoguanidine Escherichia coli MV1161 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA
-
 ?
additional information repairs 5-formyluracil, a major thymine lesion produced by reactive oxygen radicals and photosensitized oxidation, 5-hydroxymethyluracil, another major thymine methyl oxidation product, is not a substrate Escherichia coli ?
-
 ?
additional information repairs 5-formyluracil, a major thymine lesion produced by reactive oxygen radicals and photosensitized oxidation, 5-hydroxymethyluracil, another major thymine methyl oxidation product, is not a substrate Escherichia coli MV1161 ?
-
 ?

Synonyms

Synonyms Comment Organism
AlkA protein
-
 Escherichia coli