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Literature summary for 3.2.2.21 extracted from

  • Matijasevic, Z.; Stering, A.; Niu, T.Q.; Austin-Ritchie, P.; Ludlum, D.B.
    Release of sulfur mustard-modified DNA bases by Escherichia coli 3-methyladenine DNA glycosylase II (1996), Carcinogenesis, 17, 2249-2252.
    View publication on PubMed

Application

Application Comment Organism
medicine sulfur mustard, or mustard gas, bis-(2-chloroethyl)sulfide is carcinogenic to humans, acutely toxic to the skin, repiratory tract and eyes and causes a delayed bone marrow depression, knowledge of the DNA damage caused by this agent and the cellular defenses against this damage are of fundamental importance Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alkylated DNA + H2O Escherichia coli
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3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA
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?

Organism

Organism UniProt Comment Textmining
Escherichia coli
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-
-

Purification (Commentary)

Purification (Comment) Organism
purified from an overproducing strain Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alkylated DNA + H2O
-
Escherichia coli 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA
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?
alkylated DNA + H2O calf thymus DNA Escherichia coli 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA
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?
additional information with sulfur mustard treated DNA as substrate enzyme releases 7-hydroxyethylthioethyl guanine and 3-hydroxyethylthioethyl adenine, can also release carboxyethylate purines from DNA Escherichia coli ?
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?