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Literature summary for 3.2.2.20 extracted from

  • Riazuddin, S.; Athar, A.; Ahmed, Z.; Lali, S.M.; Sohail, A.
    DNA glycosylase enzymes induced during chemical adaptation of M. luteus (1987), Nucleic Acids Res., 15, 6607-6624.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.20

General Stability

General Stability Organism
very unstable Micrococcus luteus

Inhibitors

Inhibitors Comment Organism Structure
3-Methyladenine
-
 Escherichia coli
3-Methyladenine
-
 Micrococcus luteus
CaCl2
-
 Micrococcus luteus
additional information
-
 Escherichia coli
additional information unaffected by spermine or spermidine Micrococcus luteus
N-ethylmaleimide
-
 Escherichia coli
N-ethylmaleimide
-
 Micrococcus luteus
p-mercuribenzoate
-
 Micrococcus luteus

Metals/Ions

Metals/Ions Comment Organism Structure
KCl
-
 Micrococcus luteus
Mg2+
-
 Escherichia coli
Mg2+ no obligatory requirement, but addition of 1.5 mM MgCl2 causes stimulation in the catalytic activity 2fold Micrococcus luteus
NaCl
-
 Micrococcus luteus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21100
-
-
 Escherichia coli
22000
-
 gel filtration, Svedberg equation Micrococcus luteus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alkylated DNA + H2O Micrococcus luteus cellular repair of alkylated DNA base modifications 3-methyladenine + ?
-
 ?
alkylated DNA + H2O Escherichia coli constititively expressed 3-methyladenine + ?
-
 ?
alkylated DNA + H2O Micrococcus luteus constititively expressed 3-methyladenine + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Micrococcus luteus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Micrococcus luteus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000242
-
-
 Micrococcus luteus

Storage Stability

Storage Stability Organism
-20°C, stored in 50% glycerol, undergoes complete inactivation in 3-4 weeks Micrococcus luteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alkylated DNA + H2O calf thymus DNA Escherichia coli 3-methyladenine + ?
-
 ?
alkylated DNA + H2O calf thymus DNA Micrococcus luteus 3-methyladenine + ?
-
 ?
alkylated DNA + H2O cellular repair of alkylated DNA base modifications Micrococcus luteus 3-methyladenine + ?
-
 ?
alkylated DNA + H2O constititively expressed Escherichia coli 3-methyladenine + ?
-
 ?
alkylated DNA + H2O constititively expressed Micrococcus luteus 3-methyladenine + ?
-
 ?
additional information
-
 Escherichia coli ?
-
 ?
additional information no activity on O2-methylcytosine, O2-methylthymine, O4-methylthymine or O6-methylguanine Micrococcus luteus ?
-
 ?

Synonyms

Synonyms Comment Organism
GI
-
 Micrococcus luteus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 5 min time for 50% inactivation Escherichia coli
50
-
 1.2 min time for 50% inactivation Micrococcus luteus
60
-
 heat labile, during a 2 min incubation reduces activity to less than 2% Micrococcus luteus

Cofactor

Cofactor Comment Organism Structure
additional information no absolute cofactor requirement Escherichia coli
additional information no absolute cofactor requirement Micrococcus luteus