BRENDA - Enzyme Database
show all sequences of 3.2.2.2

Purine catabolism in plants

Guranowski, A.; Plant Physiol. 70, 344-349 (1982)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.065
-
Inosine
-
Lupinus luteus
0.47
-
adenosine
-
Lupinus luteus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
62000
-
gel filtration
Lupinus luteus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
inosine + H2O
Lupinus luteus
-
hypoxanthine + D-ribose
-
Lupinus luteus
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Lupinus luteus
-
L. cv. Topaz
-
Purification (Commentary)
Commentary
Organism
partial purification
Lupinus luteus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
seed
-
Lupinus luteus
-
seedling
-
Lupinus luteus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00275
-
substrate: inosine
Lupinus luteus
Storage Stability
Storage Stability
Organism
-20°C, tolerates repeated freeze-thawing
Lupinus luteus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6-mercaptopurine riboside + H2O
-
137050
Lupinus luteus
6-mercaptopurine + D-ribose
-
137050
Lupinus luteus
?
8-azainosine + H2O
-
137050
Lupinus luteus
8-azahypoxanthine + D-ribose
-
-
-
?
adenosine + H2O
-
137050
Lupinus luteus
adenine + D-ribose
-
137050
Lupinus luteus
?
guanosine + H2O
-
137050
Lupinus luteus
guanine + D-ribose
-
137050
Lupinus luteus
?
inosine + H2O
-
137050
Lupinus luteus
hypoxanthine + D-ribose
-
137050
Lupinus luteus
?
purine riboside + H2O
nebularine
137050
Lupinus luteus
purine + D-ribose
-
137050
Lupinus luteus
?
xanthosine + H2O
-
137050
Lupinus luteus
xanthine + D-ribose
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
around pH 8
Lupinus luteus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.065
-
Inosine
-
Lupinus luteus
0.47
-
adenosine
-
Lupinus luteus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
62000
-
gel filtration
Lupinus luteus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
inosine + H2O
Lupinus luteus
-
hypoxanthine + D-ribose
-
Lupinus luteus
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial purification
Lupinus luteus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
seed
-
Lupinus luteus
-
seedling
-
Lupinus luteus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00275
-
substrate: inosine
Lupinus luteus
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, tolerates repeated freeze-thawing
Lupinus luteus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6-mercaptopurine riboside + H2O
-
137050
Lupinus luteus
6-mercaptopurine + D-ribose
-
137050
Lupinus luteus
?
8-azainosine + H2O
-
137050
Lupinus luteus
8-azahypoxanthine + D-ribose
-
-
-
?
adenosine + H2O
-
137050
Lupinus luteus
adenine + D-ribose
-
137050
Lupinus luteus
?
guanosine + H2O
-
137050
Lupinus luteus
guanine + D-ribose
-
137050
Lupinus luteus
?
inosine + H2O
-
137050
Lupinus luteus
hypoxanthine + D-ribose
-
137050
Lupinus luteus
?
purine riboside + H2O
nebularine
137050
Lupinus luteus
purine + D-ribose
-
137050
Lupinus luteus
?
xanthosine + H2O
-
137050
Lupinus luteus
xanthine + D-ribose
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
around pH 8
Lupinus luteus
Other publictions for EC 3.2.2.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
713705
Elshafei
-
Degradation of purine ribonucl ...
Penicillium viridicatum, Penicillium viridicatum NRC 3712
Afr. J. Microbiol. Res.
5
316-323
2011
-
-
-
-
-
1
7
-
-
-
-
-
-
2
-
-
-
-
-
1
-
1
6
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
7
-
-
-
-
-
-
-
-
-
-
-
1
-
1
6
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
716542
Jung
Arabidopsis nucleoside hydrola ...
Arabidopsis thaliana
Plant J.
65
703-711
2011
-
-
-
-
-
-
-
5
1
-
-
-
-
1
-
-
-
-
-
1
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
1
-
-
-
-
-
-
-
-
1
-
-
5
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
695873
Porcelli
Biochemical characterization a ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2
Arch. Biochem. Biophys.
483
55-65
2009
-
-
1
-
-
-
1
3
-
1
2
-
-
45
-
-
1
-
-
-
-
-
6
1
1
1
4
3
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
1
-
3
-
1
2
-
-
-
-
1
-
-
-
-
6
1
1
1
4
3
-
-
-
1
-
-
-
-
-
-
700741
Jung
Uridine-ribohydrolase is a key ...
Arabidopsis thaliana
Plant Cell
21
876-891
2009
-
-
1
-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
-
5
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
5
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
710272
Mach
-
Uridine ribohydrolase and the ...
Arabidopsis thaliana
Plant Cell
21
0000
2009
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
700035
Liang
The role of a purine-specific ...
Bacillus thuringiensis
Microbiology
154
1333-1340
2008
-
-
1
-
-
-
-
1
-
-
-
-
-
4
-
-
1
-
-
1
3
-
3
-
1
1
-
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
3
-
3
-
1
1
-
1
1
-
-
-
-
-
-
-
-
-
683604
Boydston
The ExsY protein is required f ...
Bacillus anthracis
J. Bacteriol.
188
7440-7448
2006
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
683973
Szuwart
Calcium-stimulated guanosine-- ...
Lupinus luteus
Phytochemistry
67
1476-1485
2006
-
-
-
-
-
-
6
2
-
1
1
-
-
1
-
-
1
-
-
3
1
-
7
1
-
-
-
-
1
-
-
-
-
-
6
-
-
-
-
-
-
-
6
6
-
2
-
1
1
-
-
-
-
1
-
3
1
-
7
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
670157
Redmond
Identification of proteins in ...
Bacillus anthracis
Microbiology
150
355-363
2004
-
-
-
-
-
-
-
-
1
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
137049
Yoshino
-
A kinetic study of inosine nuc ...
Azotobacter vinelandii
Int. J. Biochem.
20
971-975
1988
-
-
-
-
-
-
3
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
137050
Guranowski
Purine catabolism in plants ...
Lupinus luteus
Plant Physiol.
70
344-349
1982
-
-
-
-
-
-
-
2
-
-
1
1
-
2
-
-
1
-
-
2
1
1
7
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
1
-
-
-
1
-
2
1
1
7
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
137051
Le Floc'h
-
The purine nucleosidases of Je ...
Helianthus tuberosus
Phytochemistry
20
2127-2129
1981
-
-
-
-
-
-
1
2
-
-
-
1
-
1
-
-
1
-
-
1
-
2
4
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
-
1
-
1
-
2
4
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
137052
Yoshino
Inosine nucleosidase from Azot ...
Azotobacter vinelandii 0, Azotobacter vinelandii
Arch. Microbiol.
119
59-64
1978
-
-
-
-
-
-
5
3
-
-
1
2
-
4
-
-
1
-
-
-
1
-
8
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
3
-
-
1
2
-
-
-
1
-
-
1
-
8
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
137053
Koch
Some enzymes of nucleoside met ...
Escherichia coli
J. Biol. Chem.
223
535-549
1956
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-