Literature summary for 3.2.2.17 extracted from

Kang, C.S.; Son, S.; Bang, I.S.
High-level expression of T4 endonuclease V in insect cells as biologically active form (2006), J. Microbiol. Biotechnol., 16, 1583-1590.

No PubMed abstract available

Search for more literature for 3.2.2.17

Cloned(Commentary)

Cloned (Comment)	Organism
T4 denV (414 bp cDNA) encoding T4 endo V (138 amino acid) is synthesized and expressed using either an expression vector, pTriEx-4, in Escherichia coli or a baculovirus AcNPV vector, pBacPAK8, in insect cells	Tequatrovirus T4

Organism

Organism	UniProt	Comment	Textmining
Tequatrovirus T4	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
the recombinant His-Tag/T4 endo V (rHis-Tag/T4 endo V) protein expressed from bacteria is purified using one-step affinity chromatography with a HiTrap Chelating HP column	Tequatrovirus T4

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	20000 U/mg. One unit of rT4 endo V activity is defined as the amount of rT4 endo V used to completely relax 250 ng of UV-irradiated plasmid DNA in 30 min at 37°C	Tequatrovirus T4

Synonyms

Synonyms	Comment	Organism
T4 Endo V	-	Tequatrovirus T4
T4 endonuclease V	-	Tequatrovirus T4

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Release 2023.1 (January 2023)