General Stability | Organism |
---|---|
resistant to EDTA | Micrococcus luteus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000015 | - |
DNA | thymine labeled DNA | Micrococcus luteus | |
0.00028 | - |
DNA | cytosine labeled DNA | Micrococcus luteus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17500 | - |
SDS-PAGE | Micrococcus luteus |
18000 | - |
- |
Micrococcus luteus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Micrococcus luteus | only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Micrococcus luteus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Micrococcus luteus |
Storage Stability | Organism |
---|---|
stable on ice for 8 months with no loss in activity | Micrococcus luteus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA phiX174 RFI + H2O | irradiated with increasing doses of UV light | Micrococcus luteus | thymine + ? | - |
? | |
additional information | only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded | Micrococcus luteus | ? | - |
? | |
oligonucleotides + H2O | - |
Micrococcus luteus | dCMP + dTMP | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 17500-18000, single polypeptide chain | Micrococcus luteus |
Synonyms | Comment | Organism |
---|---|---|
5'dimer DNA glycosylase | - |
Micrococcus luteus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 55 | thermal inactivation of both endonuclease and glycosylase activity | Micrococcus luteus |