Literature summary for 3.2.2.17 extracted from

Nyaga, S.G.; Dodson, M.L.; Lloyd, R.S.
Role of specific amino acid residues in T4 endonuclease V that alter nontarget DNA binding (1997), Biochemistry, 36, 4080-4088.

Search for more literature for 3.2.2.17

Crystallization (Commentary)

Crystallization (Comment)	Organism
-	Tequatrovirus T4

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.000003	-	DNA	mutant Q15R, R117N	Tequatrovirus T4
0.000005	-	DNA	wild-type	Tequatrovirus T4
0.000013	-	DNA	mutant K121N	Tequatrovirus T4
0.000024	-	DNA	mutant K86N	Tequatrovirus T4

Organism

Organism	UniProt	Comment	Textmining
Tequatrovirus T4	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
site-directed mutant enzymes	Tequatrovirus T4

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DNA + H2O	beta-elimination reaction results in an alpha,beta-unsaturated aldehyde	Tequatrovirus T4	thymine + ?	-	?

Synonyms

Synonyms	Comment	Organism
denV	-	Tequatrovirus T4
T4 PD-DNA-glycosylase	-	Tequatrovirus T4

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
15	37	possibility of denaturation of the substrate at temperatures above 20°C	Tequatrovirus T4

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Release 2023.1 (January 2023)