Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.2.17 extracted from

  • Warner, H.R.; Christensen, L.M.; Persson, M.L.
    Evidence that the UV endonuclease activity induced by bacteriophage T4 contains both pyrimidine dimer-DNA glycosylase and apyrimidinic/apurinic endonuclease activities in the enzyme molecule (1981), J. Virol., 10, 204-210.
No PubMed abstract available
Search for more literature for 3.2.2.17

Inhibitors

Inhibitors Comment Organism Structure
DNA depurinated DNA competitively inhibits the UV endonuclease activity, irradiated DNA inhibits the AP endonuclease activity Micrococcus luteus

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl
-
 Tequatrovirus T4
NaCl stimulated by the presence of 50-100 mM salt Micrococcus luteus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 Stokes radius Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Micrococcus luteus only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded ?
-
 ?
additional information Tequatrovirus T4 only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded ?
-
 ?

Organism

Organism UniProt Comment Textmining
Micrococcus luteus
-
-
-
Tequatrovirus T4
-
 T4amN82, host Escherichia coli B/5
-

Purification (Commentary)

Purification (Comment) Organism
-
 Micrococcus luteus
-
 Tequatrovirus T4

Source Tissue

Source Tissue Comment Organism Textmining
infected cell cells infected with wild-type phage T4 Tequatrovirus T4
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded Micrococcus luteus ?
-
 ?
additional information only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded Tequatrovirus T4 ?
-
 ?

Synonyms

Synonyms Comment Organism
denV
-
 Tequatrovirus T4
endonuclease V
-
 Tequatrovirus T4
T4 PD-DNA-glycosylase
-
 Tequatrovirus T4
T4-induced UV endonuclease
-
 Tequatrovirus T4
UV-specific endonuclease
-
 Tequatrovirus T4

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
46
-
 2 enzyme activities resides in the same protein molecule, activities lost in parallel during incubation for up to 17 min at 46°C Tequatrovirus T4
50
-
 UV endonuclease and AP endonuclease inactivated in parallel at 50°C Micrococcus luteus