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Literature summary for 3.2.2.17 extracted from

  • Ishida, M.; Kanamori, Y.; Hori, N.; Inaoka, T.; Ohtsuka, E.
    In vitro and in vivo activities of T4 endonuclease V mutants altered in the C-terminal aromatic region (1990), Biochemistry, 29, 3817-3821.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
pEVC127, cloned and expressed in Escherichia coli HB101, synthetic gene constructed by ligation of oligonucleotide fragments expressed efficiently in Escherichia coli Tequatrovirus T4

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Tequatrovirus T4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000015
-
DNA wild-type Tequatrovirus T4
0.000015
-
DNA mutant Met-127 Tequatrovirus T4
0.0001
-
DNA mutant T127M, same Km value as the wild-type Tequatrovirus T4

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
-
-

Synonyms

Synonyms Comment Organism
PD-glycosylase
-
Tequatrovirus T4
thymine photodimer repair enzyme
-
Tequatrovirus T4