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Literature summary for 3.2.2.16 extracted from

  • Cho, Y.; Kim, J.; Kim, D.
    Cobalt-requiring 5'-deoxy-5'-methylthioadenosine nucleosidase from soybean (Glycine max) cotyledon (2012), J. Plant Biochem. Biotechnol., 21, 113-116.
No PubMed abstract available
Search for more literature for 3.2.2.16

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithio-bis(2-nitrobenzoic acid)
-
 Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Glycine max

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ requires Co2+ for activity. Cobalt is bound to the nucleosidase with a stoichiometry of 1 equivalent of cobalt/subunit Glycine max
Co2+ required for catalytic function Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59500
-
 3 * 59500, SDS-PAGE Glycine max
180000
-
 gel filtration Glycine max

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5'-deoxy-5'-methylthioadenosine + H2O Glycine max
-
 ?
-
 ?
5'-methylthioadenosine + H2O Glycine max
-
 adenine + S-methyl-5-thio-D-ribose
-
 ?

Organism

Organism UniProt Comment Textmining
Glycine max
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, acetone precipitation, Sephacryl S-200 gel filtration, DEAE-Sephacel gel filtration, and 5'-AMP-Sepharose column chromatography Glycine max
native enzyme 675.2fold from cotyledons by ammonium sulfate fractionation, acetone precipitation, gel filtration, anion exchange chromatography, and affinity chromatography Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
 Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.025
-
 crude extract, at pH 7.0 and 37°C Glycine max
16.88
-
 purified native enzyme, pH 7.0, 37°C Glycine max
16.88
-
 after 675.2fold purification, at pH 7.0 and 37°C Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-deoxy-5'-methylthioadenosine + H2O
-
 Glycine max ?
-
 ?
5'-methylthioadenosine + H2O
-
 Glycine max adenine + S-methyl-5-thio-D-ribose
-
 ?

Subunits

Subunits Comment Organism
homotrimer 3 * 59500, SDS-PAGE Glycine max
trimer 3 * 59500, SDS-PAGE Glycine max

Synonyms

Synonyms Comment Organism
5'-deoxy-5'-methylthioadenosine nucleosidase
-
 Glycine max
MTA nucleosidase
-
 Glycine max

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Glycine max