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Literature summary for 3.2.2.16 extracted from

  • Cornell, K.A.; Primus, S.; Martinez, J.A.; Parveen, N.
    Assessment of methylthioadenosine/S-adenosylhomocysteine nucleosidases of Borrelia burgdorferi as targets for novel antimicrobials using a novel high-throughput method (2009), J. Antimicrob. Chemother., 63, 1163-1172.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.16

Cloned(Commentary)

Cloned (Comment) Organism
-
 Borreliella burgdorferi

Inhibitors

Inhibitors Comment Organism Structure
(3R,4S)-4-(benzylthiomethyl)-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxypyrrolidine complete inhibition at 0.1 mM Borreliella burgdorferi
5'-p-aminophenylthioadenosine Pfs protein shows 45% relative activity in the presence of 0.1 mM 5'-p-aminophenylthioadenosine, Bgp protein shows 20% relative activity in the presence of 0.1 mM 5'-p-aminophenylthioadenosine Borreliella burgdorferi
5'-p-nitrophenylthioadenosine
-
 Borreliella burgdorferi
5'-p-nitrophenythioadenosine Pfs protein shows 0.6% relative activity in the presence of 0.1 mM 5'-p-aminophenylthioadenosine, Bgp protein shows 5% relative activity in the presence of 0.1 mM 5'-p-aminophenylthioadenosine Borreliella burgdorferi
Formycin A complete inhibition at 0.1 mM Borreliella burgdorferi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0007
-
 5'-methylthioadenosine Pfs protein, at pH 5.0 Borreliella burgdorferi
0.005
-
 5'-methylthioadenosine Bgp protein, at pH 5.0 Borreliella burgdorferi

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface Bgp protein Borreliella burgdorferi 9986
-
cytoplasm Pfs protein Borreliella burgdorferi 5737
-

Organism

Organism UniProt Comment Textmining
Borreliella burgdorferi
-
 isolate B314 and strain N40
-

Purification (Commentary)

Purification (Comment) Organism
-
 Borreliella burgdorferi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1
-
 Bgp protein, using 5'-methylthioadenosine as substrate, pH 5.0 Borreliella burgdorferi
4.4
-
 Pfs protein, using 5'-methylthioadenosine as substrate, pH 5.0 Borreliella burgdorferi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-methylthioadenosine + H2O Pfs protein and Bgp protein display 100% activity towards 5'-methylthioadenosine Borreliella burgdorferi 5-methylthio-D-ribose + adenine
-
 ?

Synonyms

Synonyms Comment Organism
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
-
 Borreliella burgdorferi
Bgp protein
-
 Borreliella burgdorferi
glycosaminoglycan-binding protein
-
 Borreliella burgdorferi
methylthioadenosine/S-adenosylhomocysteine nucleosidase
-
 Borreliella burgdorferi
MTA/SAH nucleosidase
-
 Borreliella burgdorferi
Pfs protein
-
 Borreliella burgdorferi

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000023
-
 (3R,4S)-4-(benzylthiomethyl)-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxypyrrolidine Bgp protein, at pH 5.0 Borreliella burgdorferi
0.0000034
-
 (3R,4S)-4-(benzylthiomethyl)-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxypyrrolidine Pfs protein, at pH 5.0 Borreliella burgdorferi
0.000077
-
 5'-p-aminophenylthioadenosine Pfs protein, at pH 5.0 Borreliella burgdorferi
0.000116
-
 5'-p-aminophenylthioadenosine Bgp protein, at pH 5.0 Borreliella burgdorferi
0.000587
-
 5'-p-nitrophenylthioadenosine Pfs protein, at pH 5.0 Borreliella burgdorferi
0.000698
-
 5'-p-nitrophenylthioadenosine Bgp protein, at pH 5.0 Borreliella burgdorferi
0.00105
-
 Formycin A Pfs protein, at pH 5.0 Borreliella burgdorferi
0.00269
-
 Formycin A Bgp protein, at pH 5.0 Borreliella burgdorferi