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Literature summary for 3.2.2.15 extracted from

  • Karran, P.; Lindahl, T.
    Enzymatic excision of free hypoxanthine from polydeoxynucleotides and DNA containing deoxyinosine monophosphate residues (1978), J. Biol. Chem., 253, 5877-5879.
    View publication on PubMed

General Stability

General Stability Organism
relatively sensitive to inhibition by neutral salts Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
NaCl 70% inhibited by addition of 0.1 M NaCl Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
K-12
-

Purification (Commentary)

Purification (Comment) Organism
partially Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
hypoxanthine-containing DNA + H2O = hypoxanthine + DNA containing an abasic site specific enzymatic cleavage of the glycosyl bond Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O more efficient on double-stranded than of single-stranded substrates, activity 10-15 times higher, removal of nonconventional residues from DNA Escherichia coli hypoxanthine + ?
-
?
polydeoxynucleotides + H2O
-
Escherichia coli hypoxanthine + ?
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
56
-
half-life of 3 min when heated at 56°C Escherichia coli