Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Lentilactobacillus buchneri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
2'-O-methyluridine | pH 7.0, 28°C | Lentilactobacillus buchneri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
130000 | - |
gel filtration | Lentilactobacillus buchneri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lentilactobacillus buchneri | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Lentilactobacillus buchneri |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.671 | - |
pH 7.0, 28°C | Lentilactobacillus buchneri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-O-methyladenosine + H2O | - |
Lentilactobacillus buchneri | 2-O-methylribose + adenine | - |
? | |
2'-O-methylcytidine + H2O | - |
Lentilactobacillus buchneri | 2-O-methylribose + cytosine | - |
? | |
2'-O-methylguanosine + H2O | - |
Lentilactobacillus buchneri | 2-O-methylribose + guanidine | - |
? | |
2'-O-methylinosine + H2O | - |
Lentilactobacillus buchneri | 2-O-methylribose + hypoxanthine | - |
? | |
2'-O-methyluridine + H2O | - |
Lentilactobacillus buchneri | 2-O-methylribose + uracil | - |
? | |
guanosine + H2O | - |
Lentilactobacillus buchneri | D-ribose + guanine | - |
? | |
additional information | the enzyme prefers ribonucleosides and 2'-O-methylribonucleosides as its hydrolytic substrates, but reacts weakly with 2'-deoxyribonucleosides | Lentilactobacillus buchneri | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 34000, SDS-PAGE | Lentilactobacillus buchneri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Lentilactobacillus buchneri |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | more than 80% of maximum activity | Lentilactobacillus buchneri |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.49 | - |
2'-O-methyluridine | pH 7.0, 28°C | Lentilactobacillus buchneri |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 10 | 1 h, more than 70% residual activity | Lentilactobacillus buchneri |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12 | - |
2'-O-methyluridine | pH 7.0, 28°C | Lentilactobacillus buchneri |