Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Brucella anthropi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 1 mM, 23% inhibition | Brucella anthropi | |
8-hydroxyquinoline | 1 mM, 93% inhibition | Brucella anthropi | |
Ag+ | 1 mM, 68% inhibition | Brucella anthropi | |
Cu2+ | 1 mM, 57% inhibition | Brucella anthropi | |
cytidine | 0.5 mM, 81% inhibition of activity with adenosine, 92% inhibition of activity with inosine, 91% inhibition of the activity with xanthosine, 94% inhibition of activity with guanosine | Brucella anthropi | |
EDTA | 1 mM, 46% inhibition | Brucella anthropi | |
Hg2+ | 1 mM,61% inhibition | Brucella anthropi | |
Mn2+ | 1 mM, 34% inhibition | Brucella anthropi | |
NaAsO2 | - |
Brucella anthropi | |
NaCN | - |
Brucella anthropi | |
NaF | - |
Brucella anthropi | |
NaN3 | - |
Brucella anthropi | |
uridine | 0.5 mM, 38% inhibition of activity with adenosine, 45% inhibition of activity with inosine, 35% inhibition of the activity with xanthosine, 58% inhibition of activity with guanosine | Brucella anthropi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0118 | - |
adenosine | - |
Brucella anthropi | |
0.0158 | - |
Xanthosine | - |
Brucella anthropi | |
0.0395 | - |
Inosine | - |
Brucella anthropi | |
0.0563 | - |
guanosine | - |
Brucella anthropi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, slightly enhances activity to 120% | Brucella anthropi | |
Co2+ | 3 mM, reactivation after preincubation with 8-hydroxyquinoline | Brucella anthropi | |
Zn2+ | 3 mM, reactivation after preincubation with 8-hydroxyquinoline | Brucella anthropi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
4 * 40000, SDS-PAGE | Brucella anthropi |
172000 | - |
gel filtration | Brucella anthropi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brucella anthropi | E15229 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Brucella anthropi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
90.3 | - |
- |
Brucella anthropi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-AMP + H2O | 0.67% of the activity with adenosine | Brucella anthropi | adenine + D-ribose 3-phosphate | - |
? | |
adenosine + H2O | - |
Brucella anthropi | adenine + D-ribose | - |
? | |
guanosine + H2O | 29.5% of the activity with adenosine | Brucella anthropi | guanine + D-ribose | - |
? | |
inosine + H2O | 78.9% of the activity with adenosine | Brucella anthropi | hypoxanthine + D-ribose | - |
? | |
xanthosine + H2O | 63.5% of the activity with adenosine | Brucella anthropi | xanthine + D-ribose | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 40000, SDS-PAGE | Brucella anthropi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Brucella anthropi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
pH 6.0, 30 min, stable up to | Brucella anthropi |
50 | - |
pH 6.0, 30 min, 50% loss of activity | Brucella anthropi |
60 | - |
pH 6.0, 30 min, 80% loss of activity | Brucella anthropi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
hydrolysis of guanosine | Brucella anthropi |
5.5 | - |
hydrolysis of inosine | Brucella anthropi |
6.5 | - |
hydrolysis of adenosine | Brucella anthropi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 9 | hydrolysis of purine nucleosides | Brucella anthropi |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | - |
30°C, 30 min, retains 40% of the initial activity | Brucella anthropi |
6 | 8.5 | 30°C, 30 min, enzyme retains more than 80% of the initial activity | Brucella anthropi |
9 | - |
30°C, 30 min, retains 40% of the initial activity | Brucella anthropi |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00211 | - |
cytidine | pH 7.0, 30°C | Brucella anthropi | |
0.00415 | - |
cytidine | pH 7.0, 30°C | Brucella anthropi | |
0.0112 | - |
cytidine | pH 7.0, 30°C | Brucella anthropi | |
0.016 | - |
cytidine | pH 7.0, 30°C | Brucella anthropi |