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Literature summary for 3.2.1.B43 extracted from

  • Nakazawa, K.; Suzuki, S.
    Purification of keratan sulfate-endogalactosidase and its action on keratan sulfates of different origin (1975), J. Biol. Chem., 250, 912-917 .
    View publication on PubMed
Search for more literature for 3.2.1.B43

Activating Compound

Activating Compound Comment Organism Structure
acetate 0.05 M, about 15% activation Pseudomonas sp. IFO-13309
Maleate 0.05 M, about 15% activation Pseudomonas sp. IFO-13309

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. IFO-13309
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp. IFO-13309

Storage Stability

Storage Stability Organism
0°C, ice bath, stable for 1 year Pseudomonas sp. IFO-13309

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
corneal keratan sulfate + H2O compared with cornea keratan sulfate, keratan sulfates from human nucleus pulposus and shark cartilage are attacked at lower rates with a resultant production of oligosaccharides of relatively large size. The point of enzyme attack is limited to the endo-beta-D-galactoside bonds in which nonsulfated D-galactose residues participate. The bond susceptible to hydrolysis is associated with beta-D-Gal-(1->4)-2-acetamido-2-deoxy-6-O-sulfo-n-glucose in keratan sulfate. The major oligosaccharide products in the digest are terminated by galactose and 2-acetamido-2-deoxy-6-O-sulfoglucose at the reducing and nonreducing end, respectively Pseudomonas sp. IFO-13309 ?
-
 ?
additional information the enzyme does not catalyze the hydrolysis of phenyl beta-D-galactoside. beta-D-Gal-(1->3)-2-acetamido-2-deoxy-beta-D-Glc-(1->3)-beta-D-Gal-(1->4)-D-Glc is completely refractory to the action of this enzyme Pseudomonas sp. IFO-13309 ?
-
 ?

Synonyms

Synonyms Comment Organism
keratan sulfate-endogalactosidase
-
 Pseudomonas sp. IFO-13309

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Pseudomonas sp. IFO-13309

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 45 30°C: about 80% of maximal activity, 45°C: about 50% of maximal activity Pseudomonas sp. IFO-13309

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 7.4
-
 Pseudomonas sp. IFO-13309