Literature summary for 3.2.1.B43 extracted from

Ohmae, M.; Sakaguchi, K.; Kaneto, T.; Fujikawa, S.; Kobayashi, S.
Keratanase II-catalyzed synthesis of keratan sulfate oligomers by using sugar oxazolines as transition-state analogue substrate monomers a novel insight into the enzymatic catalysis mechanism (2017), Chembiochem, 8, 1710-1720 .

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Organism

Organism	UniProt	Comment	Textmining
Bacillus sp. Ks36	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	transglycosylation to form beta-(1->3)-glycosidic bond through a substrate-assisted mechanism. N-Acetyllactosamine oxazoline derivatives with sulfate groups at the C-6 are effectively oligomerized by the enzyme under weak alkaline conditions, to give alternating 6-sulfated keratan sulfate oligomers in good yields. The enzyme also recognized N-acetyllactosamine oxazoline derivatives with sulfate groups at both the C-6 and the C-6', which provides fully 6-sulfated KS oligomers in good yields under similar conditions. Nonsulfated LacNAc oxazoline is difficult to oligomerize enzymatically. The catalysis mechanism of KSase II involves a sugar oxazolinium ion that requires the 6-sulfate group in the GlcNAc residue not only in hydrolysis of keratan sulfate chains, but also in oligomerization of oxazoline monomers	Bacillus sp. Ks36	?	-	?

Synonyms

Synonyms	Comment	Organism
keratanase II	-	Bacillus sp. Ks36
KSase II	-	Bacillus sp. Ks36

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Release 2023.1 (January 2023)