Cloned (Comment) | Organism |
---|---|
expression in Streptomyces lividans and Escherichia coli | Thermobifida fusca |
Protein Variants | Comment | Organism |
---|---|---|
D55C | mutation in proposed catalytic site, low activity, mutant is able to bind bacterial microcrystalline cellulose | Thermobifida fusca |
additional information | Removal of the internal family IIIc CBD decreases activity markedly on every substrate. Constructs which lack the family IIIc CBD have almost no activity on bacterial microcrystalline cellulose | Thermobifida fusca |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90400 | - |
calculated from sequence | Thermobifida fusca |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermobifida fusca | P26221 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxymethyl cellulose + H2O | - |
Thermobifida fusca | cellotetraose + ? | - |
? | |
cellulose + H2O | bacterial microcrystalline cellulose | Thermobifida fusca | cellotetraose + ? | - |
? | |
filter paper + H2O | - |
Thermobifida fusca | cellotetraose + ? | - |
? | |
swollen cellulose paper + H2O | - |
Thermobifida fusca | cellotetraose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 90400, calculated from sequence | Thermobifida fusca |
Synonyms | Comment | Organism |
---|---|---|
CelD | - |
Thermobifida fusca |
E4 | - |
Thermobifida fusca |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
56 | - |
16 h, 50% of the activity is retained for constructions lacking the type IIIc CBD | Thermobifida fusca |
63 | - |
16 h, 50% of the activity is retained for constructions containing the type IIIc CBD | Thermobifida fusca |
General Information | Comment | Organism |
---|---|---|
physiological function | enzyme is comprised of a catalytic domain (CD), an internal family IIIc cellulose binding domain (CBD), a fibronectinlike domain, and a family II CBD. Removal of the internal family IIIc CBD decreases activity markedly on every substrate | Thermobifida fusca |