Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.B42 extracted from

  • Irwin, D.; Shin,D.-H.; Zhang, S.; Barr,b.K.; Sakon,j.; Karplus, P.A.; Wilson, D.B.
    Roles of the catalytic domain and two cellulose binding domains of Thermomonospora fusca E4 in cellulose hydrolysis (1998), J. Bacteriol., 180, 1709-1714 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Streptomyces lividans and Escherichia coli Thermobifida fusca

Protein Variants

Protein Variants Comment Organism
D55C mutation in proposed catalytic site, low activity, mutant is able to bind bacterial microcrystalline cellulose Thermobifida fusca
additional information Removal of the internal family IIIc CBD decreases activity markedly on every substrate. Constructs which lack the family IIIc CBD have almost no activity on bacterial microcrystalline cellulose Thermobifida fusca

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90400
-
calculated from sequence Thermobifida fusca

Organism

Organism UniProt Comment Textmining
Thermobifida fusca P26221
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethyl cellulose + H2O
-
Thermobifida fusca cellotetraose + ?
-
?
cellulose + H2O bacterial microcrystalline cellulose Thermobifida fusca cellotetraose + ?
-
?
filter paper + H2O
-
Thermobifida fusca cellotetraose + ?
-
?
swollen cellulose paper + H2O
-
Thermobifida fusca cellotetraose + ?
-
?

Subunits

Subunits Comment Organism
? x * 90400, calculated from sequence Thermobifida fusca

Synonyms

Synonyms Comment Organism
CelD
-
Thermobifida fusca
E4
-
Thermobifida fusca

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
56
-
16 h, 50% of the activity is retained for constructions lacking the type IIIc CBD Thermobifida fusca
63
-
16 h, 50% of the activity is retained for constructions containing the type IIIc CBD Thermobifida fusca

General Information

General Information Comment Organism
physiological function enzyme is comprised of a catalytic domain (CD), an internal family IIIc cellulose binding domain (CBD), a fibronectinlike domain, and a family II CBD. Removal of the internal family IIIc CBD decreases activity markedly on every substrate Thermobifida fusca