BRENDA - Enzyme Database
show all sequences of 3.2.1.B41

Gene cloning and enzymatic properties of hyperthermostable beta-glycosidase from Thermus thermophilus HJ6

Gu, N.; Kim, J.; Kim, H.; You, D.; Kim, H.; Jeon, S.; J. Biosci. Bioeng. 107, 21-26 (2009)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli
Thermus thermophilus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
4-nitrophenyl beta-D-glucopyranoside
Km-values decrease with an increase in temperature, whereas the kcat values increase, so the catalytic efficiency (kcat/Km) increases 70fold between 40°C and 80°C
Thermus thermophilus
0.08
-
4-nitrophenyl beta-D-fucopyranoside
80°C, pH 8.5
Thermus thermophilus
0.16
-
4-nitrophenyl beta-D-glucopyranoside
80°C, pH 8.5
Thermus thermophilus
0.28
-
4-nitrophenyl beta-D-glucopyranoside
70°C, pH 8.5
Thermus thermophilus
0.39
-
4-nitrophenyl beta-D-glucopyranoside
60°C, pH 8.5
Thermus thermophilus
0.83
-
4-nitrophenyl beta-D-glucopyranoside
50°C, pH 8.5
Thermus thermophilus
1.74
-
4-nitrophenyl beta-D-galactopyranoside
80°C, pH 8.5
Thermus thermophilus
3.76
-
4-nitrophenyl beta-D-glucopyranoside
40°C, pH 8.5
Thermus thermophilus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
-
Thermus thermophilus
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
SDS-PAGE
Thermus thermophilus
48700
-
calculated from sequence
Thermus thermophilus
50000
-
gel filtration
Thermus thermophilus
Organism
Organism
UniProt
Commentary
Textmining
Thermus thermophilus
A8WAC9
-
-
Thermus thermophilus HJ6
A8WAC9
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Thermus thermophilus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
4-nitrophenyl beta-D-fucopyranoside + H2O
-
748250
Thermus thermophilus
4-nitrophenol + D-fucose
-
-
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
-
748250
Thermus thermophilus HJ6
4-nitrophenol + D-fucose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
-
748250
Thermus thermophilus
4-nitrophenol + D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
-
748250
Thermus thermophilus HJ6
4-nitrophenol + D-galactose
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
748250
Thermus thermophilus
4-nitrophenol + D-glucose
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
748250
Thermus thermophilus HJ6
4-nitrophenol + D-glucose
-
-
-
?
additional information
the enzyme has transglycosylation activity at high temperature and that various transfer products were formed in the reaction with lactose or cellobiose
748250
Thermus thermophilus
?
-
-
-
-
additional information
the enzyme has transglycosylation activity at high temperature and that various transfer products were formed in the reaction with lactose or cellobiose
748250
Thermus thermophilus HJ6
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 45000, SDS-PAGE
Thermus thermophilus
Synonyms
Synonyms
Commentary
Organism
TtbetaGly
-
Thermus thermophilus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
90
-
-
Thermus thermophilus
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
70
105
70°C: about 55% of maximal activity, 105°C: about 60% of maximal activity
Thermus thermophilus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
95
-
half-life: 30 min
Thermus thermophilus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
4-nitrophenyl beta-D-glucopyranoside
Km-values decrease with an increase in temperature, whereas the kcat values increase, so the catalytic efficiency (kcat/Km) increases 70fold between 40°C and 80°C
Thermus thermophilus
3.1
-
4-nitrophenyl beta-D-glucopyranoside
40°C, pH 8.5
Thermus thermophilus
3.17
-
4-nitrophenyl beta-D-glucopyranoside
50°C, pH 8.5
Thermus thermophilus
4.78
-
4-nitrophenyl beta-D-glucopyranoside
60°C, pH 8.5
Thermus thermophilus
7.56
-
4-nitrophenyl beta-D-glucopyranoside
70°C, pH 8.5
Thermus thermophilus
8.94
-
4-nitrophenyl beta-D-fucopyranoside
80°C, pH 8.5
Thermus thermophilus
9.24
-
4-nitrophenyl beta-D-glucopyranoside
80°C, pH 8.5
Thermus thermophilus
9.64
-
4-nitrophenyl beta-D-galactopyranoside
80°C, pH 8.5
Thermus thermophilus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Thermus thermophilus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
8
10
pH 8.0: about 40% of maximal activity, pH 10.0: about 70% of maximal activity
Thermus thermophilus
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Thermus thermophilus
calculated from sequence
-
5.6
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli
Thermus thermophilus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
4-nitrophenyl beta-D-glucopyranoside
Km-values decrease with an increase in temperature, whereas the kcat values increase, so the catalytic efficiency (kcat/Km) increases 70fold between 40°C and 80°C
Thermus thermophilus
0.08
-
4-nitrophenyl beta-D-fucopyranoside
80°C, pH 8.5
Thermus thermophilus
0.16
-
4-nitrophenyl beta-D-glucopyranoside
80°C, pH 8.5
Thermus thermophilus
0.28
-
4-nitrophenyl beta-D-glucopyranoside
70°C, pH 8.5
Thermus thermophilus
0.39
-
4-nitrophenyl beta-D-glucopyranoside
60°C, pH 8.5
Thermus thermophilus
0.83
-
4-nitrophenyl beta-D-glucopyranoside
50°C, pH 8.5
Thermus thermophilus
1.74
-
4-nitrophenyl beta-D-galactopyranoside
80°C, pH 8.5
Thermus thermophilus
3.76
-
4-nitrophenyl beta-D-glucopyranoside
40°C, pH 8.5
Thermus thermophilus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
-
Thermus thermophilus
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
SDS-PAGE
Thermus thermophilus
48700
-
calculated from sequence
Thermus thermophilus
50000
-
gel filtration
Thermus thermophilus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Thermus thermophilus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
4-nitrophenyl beta-D-fucopyranoside + H2O
-
748250
Thermus thermophilus
4-nitrophenol + D-fucose
-
-
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
-
748250
Thermus thermophilus HJ6
4-nitrophenol + D-fucose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
-
748250
Thermus thermophilus
4-nitrophenol + D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
-
748250
Thermus thermophilus HJ6
4-nitrophenol + D-galactose
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
748250
Thermus thermophilus
4-nitrophenol + D-glucose
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
748250
Thermus thermophilus HJ6
4-nitrophenol + D-glucose
-
-
-
?
additional information
the enzyme has transglycosylation activity at high temperature and that various transfer products were formed in the reaction with lactose or cellobiose
748250
Thermus thermophilus
?
-
-
-
-
additional information
the enzyme has transglycosylation activity at high temperature and that various transfer products were formed in the reaction with lactose or cellobiose
748250
Thermus thermophilus HJ6
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 45000, SDS-PAGE
Thermus thermophilus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
90
-
-
Thermus thermophilus
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
70
105
70°C: about 55% of maximal activity, 105°C: about 60% of maximal activity
Thermus thermophilus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
95
-
half-life: 30 min
Thermus thermophilus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
4-nitrophenyl beta-D-glucopyranoside
Km-values decrease with an increase in temperature, whereas the kcat values increase, so the catalytic efficiency (kcat/Km) increases 70fold between 40°C and 80°C
Thermus thermophilus
3.1
-
4-nitrophenyl beta-D-glucopyranoside
40°C, pH 8.5
Thermus thermophilus
3.17
-
4-nitrophenyl beta-D-glucopyranoside
50°C, pH 8.5
Thermus thermophilus
4.78
-
4-nitrophenyl beta-D-glucopyranoside
60°C, pH 8.5
Thermus thermophilus
7.56
-
4-nitrophenyl beta-D-glucopyranoside
70°C, pH 8.5
Thermus thermophilus
8.94
-
4-nitrophenyl beta-D-fucopyranoside
80°C, pH 8.5
Thermus thermophilus
9.24
-
4-nitrophenyl beta-D-glucopyranoside
80°C, pH 8.5
Thermus thermophilus
9.64
-
4-nitrophenyl beta-D-galactopyranoside
80°C, pH 8.5
Thermus thermophilus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Thermus thermophilus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
8
10
pH 8.0: about 40% of maximal activity, pH 10.0: about 70% of maximal activity
Thermus thermophilus
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Thermus thermophilus
calculated from sequence
-
5.6
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
additional information
-
4-nitrophenyl beta-D-glucopyranoside
Km-values decrease with an increase in temperature, whereas the kcat values increase, so the catalytic efficiency (kcat/Km) increases 70fold between 40°C and 80°C
Thermus thermophilus
0.82
-
4-nitrophenyl beta-D-glucopyranoside
40°C, pH 8.5
Thermus thermophilus
3.83
-
4-nitrophenyl beta-D-glucopyranoside
50°C, pH 8.5
Thermus thermophilus
5.56
-
4-nitrophenyl beta-D-galactopyranoside
80°C, pH 8.5
Thermus thermophilus
12.22
-
4-nitrophenyl beta-D-glucopyranoside
60°C, pH 8.5
Thermus thermophilus
27.45
-
4-nitrophenyl beta-D-glucopyranoside
70°C, pH 8.5
Thermus thermophilus
57.12
-
4-nitrophenyl beta-D-glucopyranoside
80°C, pH 8.5
Thermus thermophilus
104.83
-
4-nitrophenyl beta-D-fucopyranoside
80°C, pH 8.5
Thermus thermophilus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
additional information
-
4-nitrophenyl beta-D-glucopyranoside
Km-values decrease with an increase in temperature, whereas the kcat values increase, so the catalytic efficiency (kcat/Km) increases 70fold between 40°C and 80°C
Thermus thermophilus
0.82
-
4-nitrophenyl beta-D-glucopyranoside
40°C, pH 8.5
Thermus thermophilus
3.83
-
4-nitrophenyl beta-D-glucopyranoside
50°C, pH 8.5
Thermus thermophilus
5.56
-
4-nitrophenyl beta-D-galactopyranoside
80°C, pH 8.5
Thermus thermophilus
12.22
-
4-nitrophenyl beta-D-glucopyranoside
60°C, pH 8.5
Thermus thermophilus
27.45
-
4-nitrophenyl beta-D-glucopyranoside
70°C, pH 8.5
Thermus thermophilus
57.12
-
4-nitrophenyl beta-D-glucopyranoside
80°C, pH 8.5
Thermus thermophilus
104.83
-
4-nitrophenyl beta-D-fucopyranoside
80°C, pH 8.5
Thermus thermophilus
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748250
Gu
Gene cloning and enzymatic pr ...
Thermus thermophilus, Thermus thermophilus HJ6
J. Biosci. Bioeng.
107
21-26
2009
-
-
1
-
-
-
-
8
1
-
3
-
-
5
-
-
1
-
-
-
-
-
8
1
1
1
1
1
8
1
1
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
8
1
-
3
-
-
-
-
1
-
-
-
-
8
1
1
1
1
8
1
1
-
1
-
-
-
-
8
8
747918
Fourage
Kinetic study of a thermostab ...
Thermus thermophilus
Glycoconj. J.
17
377-383
2000
-
-
1
-
-
-
1
4
-
-
-
-
-
3
-
-
1
-
-
-
-
-
6
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
1
-
-
-
-
6
-
-
-
-
3
-
-
-
-
-
-
-
-
3
3