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Literature summary for 3.2.1.B41 extracted from

  • Fourage, L.; Dion, M.; Colas, B.
    Kinetic study of a thermostable beta-glycosidase of Thermus thermophilus. Effects of temperature and glucose on hydrolysis and transglycosylation reactions (2000), Glycoconj. J., 17, 377-383 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli Thermus thermophilus

Inhibitors

Inhibitors Comment Organism Structure
additional information the substrate can act not only as a glycosyl donor but also as a glycosyl acceptor. In addition, when the glucose is added to reaction mixtures, inhibition or activation is observed depending on both substrate concentration and temperature. A reaction model is proposed to explain the kinetic behavior of the enzyme. The scheme integrates the inhibition observed at high concentrations of substrate and the activation due to transglycosylation reactions implicating the existence of a transfer subsite Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information when the kinetics are performed below 60°C, the enzyme is inhibited by high substrate concentrations. At temperatures higher than 60°C, the inhibition phenomenon is no longer observed but, on the contrary, an activation is obtained at high substrate concentrations. Around 60°C, the enzyme displays Michaelian behavior Thermus thermophilus
0.1
-
4-nitrophenyl beta-D-glucoside 60°C, pH 7.0 Thermus thermophilus
0.12
-
4-nitrophenyl beta-D-fucoside 60°C, pH 7.0 Thermus thermophilus
5.6
-
4-nitrophenyl beta-D-galactoside 60°C, pH 7.0 Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-cellobioside + H2O
-
Thermus thermophilus 4-nitrophenyl beta-D-glucoside + D-glucose
-
?
4-nitrophenyl beta-D-fucoside + H2O
-
Thermus thermophilus 4-nitrophenol + beta-D-fucose
-
?
4-nitrophenyl beta-D-galactoside + H2O
-
Thermus thermophilus 4-nitrophenol + D-galactose
-
?
4-nitrophenyl beta-D-glucoside + H2O
-
Thermus thermophilus 4-nitrophenol + D-glucose
-
?
cellotetraose + H2O
-
Thermus thermophilus cellotriose + D-glucose
-
?
additional information the enzyme is highly specific for beta-linked sugars. The substrate can act not only as a glycosyl donor but also as a glycosyl acceptor. In addition, when the glucose is added to reaction mixtures, inhibition or activation is observed depending on both substrate concentration and temperature. A reaction model is proposed to explain the kinetic behavior of the enzyme. The scheme integrates the inhibition observed at high concentrations of substrate and the activation due to transglycosylation reactions implicating the existence of a transfer subsite Thermus thermophilus ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
23.4
-
4-nitrophenyl beta-D-glucoside 60°C, pH 7.0 Thermus thermophilus
29.7
-
4-nitrophenyl beta-D-fucoside 60°C, pH 7.0 Thermus thermophilus
82.9
-
4-nitrophenyl beta-D-galactoside 60°C, pH 7.0 Thermus thermophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
14.8
-
4-nitrophenyl beta-D-galactoside 60°C, pH 7.0 Thermus thermophilus
227
-
4-nitrophenyl beta-D-glucoside 60°C, pH 7.0 Thermus thermophilus
247
-
4-nitrophenyl beta-D-fucoside 60°C, pH 7.0 Thermus thermophilus