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Literature summary for 3.2.1.B33 extracted from
- Enzymatic characterization and substrate specificity of thermostable beta-glycosidase from hyperthermophilic archaea, Sulfolobus shibatae, expressed in Escherichia coli (2007), J. Microbiol. Biotechnol., 17, 454-460.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | increases of the hydrolysis activity | Saccharolobus shibatae |  |
| dithiothreitol | increases the hydrolysis activity | Saccharolobus shibatae | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Saccharolobus shibatae |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | no effect by 1-5 mM. Addition of 10 mM of MnCl2 enhances the hydrolysis activity of rSSG up to 23% whereas most metal ions do not show any considerable effect | Saccharolobus shibatae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
4 * 57000, SDS-PAGE | Saccharolobus shibatae |
| 240000 | - |
gel filtration | Saccharolobus shibatae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus shibatae | - |
- |
- |
| Saccharolobus shibatae JCM 8931 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharolobus shibatae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellobiose + H2O | the enzyme prefers laminaribiose (beta1->3Glc), followed by sophorose (beta1->2Glc), gentiobiose (beta1->6Glc), and cellobiose (beta1->4Glc) | Saccharolobus shibatae | D-glucose + D-glucose | - |
? | |
| cellobiose + H2O | the enzyme prefers laminaribiose (beta1->3Glc), followed by sophorose (beta1->2Glc), gentiobiose (beta1->6Glc), and cellobiose (beta1->4Glc) | Saccharolobus shibatae JCM 8931 | D-glucose + D-glucose | - |
? | |
| gentiobiose + H2O | the enzyme prefers laminaribiose (beta1->3Glc), followed by sophorose (beta1->2Glc), gentiobiose (beta1->6Glc), and cellobiose (beta1->4Glc) | Saccharolobus shibatae | D-glucose + D-glucose | - |
? | |
| gentiobiose + H2O | the enzyme prefers laminaribiose (beta1->3Glc), followed by sophorose (beta1->2Glc), gentiobiose (beta1->6Glc), and cellobiose (beta1->4Glc) | Saccharolobus shibatae JCM 8931 | D-glucose + D-glucose | - |
? | |
| lactose + H2O | strong intermolecular transglycosylation activity. Various intermolecular transfer products are formed, indicating that the enzyme prefers lactose as a good acceptor as well as a donor | Saccharolobus shibatae | D-glucose + beta-D-galactose | - |
? | |
| lactose + H2O | strong intermolecular transglycosylation activity. Various intermolecular transfer products are formed, indicating that the enzyme prefers lactose as a good acceptor as well as a donor | Saccharolobus shibatae JCM 8931 | D-glucose + beta-D-galactose | - |
? | |
| laminaribiose + H2O | the enzyme prefers laminaribiose (beta1->3Glc), followed by sophorose (beta1->2Glc), gentiobiose (beta1->6Glc), and cellobiose (beta1->4Glc) | Saccharolobus shibatae | D-glucose + D-glucose | - |
? | |
| laminaribiose + H2O | the enzyme prefers laminaribiose (beta1->3Glc), followed by sophorose (beta1->2Glc), gentiobiose (beta1->6Glc), and cellobiose (beta1->4Glc) | Saccharolobus shibatae JCM 8931 | D-glucose + D-glucose | - |
? | |
| sophorose + H2O | the enzyme prefers laminaribiose (beta1->3Glc), followed by sophorose (beta1->2Glc), gentiobiose (beta1->6Glc), and cellobiose (beta1->4Glc) | Saccharolobus shibatae | D-glucose + D-glucose | - |
? | |
| sophorose + H2O | the enzyme prefers laminaribiose (beta1->3Glc), followed by sophorose (beta1->2Glc), gentiobiose (beta1->6Glc), and cellobiose (beta1->4Glc) | Saccharolobus shibatae JCM 8931 | D-glucose + D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 57000, SDS-PAGE | Saccharolobus shibatae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Sulfolobus shibatae beta-glycosidase | - |
Saccharolobus shibatae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
- |
Saccharolobus shibatae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | 100 | 80°C: about 70% of maximal activity, 100°C: about 80% of maximal activity | Saccharolobus shibatae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
half-life: 15 h | Saccharolobus shibatae |
| 95 | - |
half-life: 3.9 min | Saccharolobus shibatae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Saccharolobus shibatae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | pH 4.0: apout 5% of maximal activity, pH 5.0: maximal activity, pH 7.0: about 40% of maximal activity | Saccharolobus shibatae |
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