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Literature summary for 3.2.1.B33 extracted from
- Thermostable beta-glucosidase with a broad substrate specificity suitable for processing of lactose-containing products (2004), Food Chem., 85, 181-187.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| phenylmethanesulfonyl fluoride | 5 mM, 1.1fold activation | Saccharolobus shibatae |  |
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | processing of lactose-containing products | Saccharolobus shibatae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 5 mM, no inhibition of 2-nitrophenyl beta-D-galactopyranoside hydrolysis, 5% inhibition of 4-nitrophenyl beta-D-glucopyranoside hydrolysis | Saccharolobus shibatae | |
| 4-chloromercuribenzoate | 5 mM, 94% inhibition of 2-nitrophenyl beta-D-galactopyranoside hydrolysis, 90% inhibition of 4-nitrophenyl beta-D-glucopyranoside hydrolysis | Saccharolobus shibatae | |
| Ba2+ | 1 mM, 5% inhibition of 2-nitrophenyl beta-D-galactopyranoside hydrolysis, 6% inhibition of 4-nitrophenyl beta-D-glucopyranoside hydrolysis | Saccharolobus shibatae | |
| Ca2+ | 1 mM, 5% inhibition of 2-nitrophenyl beta-D-galactopyranoside hydrolysis, 6% inhibition of 4-nitrophenyl beta-D-glucopyranoside hydrolysis | Saccharolobus shibatae | |
| Cd2+ | no inhibition of 2-nitrophenyl beta-D-galactopyranoside hydrolysis, 12% inhibition of 4-nitrophenyl beta-D-glucopyranoside hydrolysis | Saccharolobus shibatae | |
| Cu2+ | 1 mM, 81% inhibition of 2-nitrophenyl beta-D-galactopyranoside hydrolysis, 36% inhibition of 4-nitrophenyl beta-D-glucopyranoside hydrolysis | Saccharolobus shibatae | |
| Fe2+ | 1 mM, 1% inhibition of 2-nitrophenyl mbeta-D-galactopyranoside hydrolysis, 16% inhibition of 4-nitrophenyl beta-D-glucopyranoside hydrolysis | Saccharolobus shibatae | |
| Hg2+ | 1 mM, complete inhibition of 2-nitrophenyl beta-D-galactopyranoside hydrolysis and 4-nitrophenyl beta-D-glucopyranoside hydrolysis | Saccharolobus shibatae | |
| monoiodoacetate | 5 mM, 3% inhibition of 2-nitrophenyl beta-D-galactopyranoside hydrolysis, no inhibition of 4-nitrophenyl beta-D-glucopyranoside hydrolysis | Saccharolobus shibatae | |
| Zn2+ | 1 mM, complete inhibition of 2-nitrophenyl beta-D-galactopyranoside hydrolysis and 4-nitrophenyl beta-D-glucopyranoside hydrolysis | Saccharolobus shibatae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Saccharolobus shibatae | 5737 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus shibatae | - |
- |
- |
| Saccharolobus shibatae DSM 5389 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharolobus shibatae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Saccharolobus shibatae | 2-nitrophenol + beta-D-galactopyranose | - |
? | |
| 2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Saccharolobus shibatae DSM 5389 | 2-nitrophenol + beta-D-galactopyranose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | rate of hydrolysis is 43.8% relative to 2-nitrophenyl-beta-D-galactopyranoside | Saccharolobus shibatae | 4-nitrophenol + D-glucopyranose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | rate of hydrolysis is 43.8% relative to 2-nitrophenyl-beta-D-galactopyranoside | Saccharolobus shibatae DSM 5389 | 4-nitrophenol + D-glucopyranose | - |
? | |
| cellobiose + H2O | rate of hydrolysis is 25.4% relative to 2-nitrophenyl-beta-D-galactopyranoside | Saccharolobus shibatae | D-glucose + D-glucose | - |
? | |
| cellobiose + H2O | rate of hydrolysis is 25.4% relative to 2-nitrophenyl-beta-D-galactopyranoside | Saccharolobus shibatae DSM 5389 | D-glucose + D-glucose | - |
? | |
| lactose + H2O | rate of hydrolysis is 15.7% relative to 2-nitrophenyl-beta-D-galactopyranoside | Saccharolobus shibatae | D-glucose + D-galactose | - |
? | |
| lactose + H2O | rate of hydrolysis is 15.7% relative to 2-nitrophenyl-beta-D-galactopyranoside | Saccharolobus shibatae DSM 5389 | D-glucose + D-galactose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
assay at | Saccharolobus shibatae |
| 98 | - |
hydrolysis of 2-nitrophenyl beta-D-galactopyranoside or 4-nitrophenyl beta-D-glucopyranoside | Saccharolobus shibatae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
the enzyme retains 50% of the initial activity towards 2-nitrophenyl beta-D-galactopyranoside or 4-nitrophenyl beta-D-glucopyranoside after 146 h and 127 h of incubation in phosphate (pH 7.2) or acetate (pH 5.5) buffers. The half-lifes of the enzyme activity at this temperature are decreased after preincubation in acetate buffer (pH 5.5) up to 32 and 27 h, respectively | Saccharolobus shibatae |
| 80 | - |
5 h, in 0.1 M acetate buffer (pH 5.5), the enzyme retained about 67% of the initial activity | Saccharolobus shibatae |
| 98 | - |
half-life at pH 7.2: 54 min, half-life at pH 5.5: 30 min | Saccharolobus shibatae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Saccharolobus shibatae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.2 | 6.9 | about 80% of maximal activity | Saccharolobus shibatae |
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