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Literature summary for 3.2.1.B28 extracted from

  • Shin, K.C.; Nam, H.K.; Oh, D.K.
    Hydrolysis of flavanone glycosides by beta-glucosidase from Pyrococcus furiosus and its application to the production of flavanone aglycones from citrus extracts. (2013), J. Agric. Food Chem., 61, 11532-11540.
    View publication on PubMed

Application

Application Comment Organism
additional information the enzyme converts flavanone glycoside to flavanone aglycone via a one-step reaction. It may be useful in the production of the flavanone aglycones naringenin and hesperetin from flavanone glycosides in citrus extracts Pyrococcus furiosus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus furiosus

Inhibitors

Inhibitors Comment Organism Structure
1-methyl-1-butanol 10% (v/v), about% inhibition Pyrococcus furiosus
2-propanol 10% (v/v), about% inhibition Pyrococcus furiosus
acetone 10% (v/v), about% inhibition Pyrococcus furiosus
DMSO 10% (v/v), about% inhibition Pyrococcus furiosus
ethanol 10% (v/v), about 50% inhibition Pyrococcus furiosus
formate 10% (v/v), about% inhibition Pyrococcus furiosus
Isopropanol 10% (v/v), about% inhibition Pyrococcus furiosus
methanol 10% (v/v), about% inhibition Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6
-
hesperidin pH 5.5, 95°C Pyrococcus furiosus
1.7
-
naringin pH 5.5, 95°C Pyrococcus furiosus
3.7
-
narirutin pH 5.5, 95°C Pyrococcus furiosus
4.6
-
neohesperidin pH 5.5, 95°C Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55488
-
x * 55488, calculated from sequence Pyrococcus furiosus
56000
-
x * 56000, SDS-PAGE Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q51723
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.011
-
substrate: rutin, pH 5.5, 95°C Pyrococcus furiosus
0.081
-
substrate: neoponcirin, pH 5.5, 95°C Pyrococcus furiosus
0.085
-
substrate: diosmin, pH 5.5, 95°C Pyrococcus furiosus
0.091
-
substrate: poncirin, pH 5.5, 95°C Pyrococcus furiosus
0.102
-
substrate naringin, pH 5.5, 95°C Pyrococcus furiosus
0.103
-
substrate: neohesperidin, pH 5.5, 95°C Pyrococcus furiosus
0.153
-
substrate: narirutin, pH 5.5, 95°C Pyrococcus furiosus
0.172
-
substrate: hesperidin, pH 5.5, 95°C Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diosmin + H2O
-
Pyrococcus furiosus diosmetin + rutinose
-
?
hesperidin + H2O
-
Pyrococcus furiosus hesperetin + rutinose
-
?
naringin + H2O
-
Pyrococcus furiosus naringenin + rutinose
-
?
narirutin + H2O
-
Pyrococcus furiosus naringenin + neohesperidose
-
?
neohesperidin + H2O
-
Pyrococcus furiosus hesperetin + neohesperidose
-
?
neoponcirin + H2O
-
Pyrococcus furiosus isosakuranetin + rutinose
-
?
poncirin + H2O
-
Pyrococcus furiosus isosakuranetin + neohesperidose
-
?
rutin + H2O
-
Pyrococcus furiosus quercetin + rutinose
-
?

Subunits

Subunits Comment Organism
? x * 56000, SDS-PAGE Pyrococcus furiosus
? x * 55488, calculated from sequence Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
-
Pyrococcus furiosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
75 95 the activity increases from 75°C to 95°C, activity at 75°C is about 40% of the activity at 95°C, substrate: hesperidin Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
half-life: 637 h Pyrococcus furiosus
80
-
half-life: 392 h Pyrococcus furiosus
85
-
half-life: 235 h Pyrococcus furiosus
90
-
half-life: 138 h Pyrococcus furiosus
95
-
half-life: 88 h Pyrococcus furiosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
27.7
-
naringin pH 5.5, 95°C Pyrococcus furiosus
68.4
-
hesperidin pH 5.5, 95°C Pyrococcus furiosus
74.1
-
narirutin pH 5.5, 95°C Pyrococcus furiosus
84.8
-
neohesperidin pH 5.5, 95°C Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
substrate: hesperidin Pyrococcus furiosus

pH Range

pH Minimum pH Maximum Comment Organism
4.5 6.5 pH 4.5: about 50% of maximal activity, pH 6.5: about 60% of maximal activity, substrate: hesperidin Pyrococcus furiosus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
16.5
-
naringin pH 5.5, 95°C Pyrococcus furiosus
18.5
-
neohesperidin pH 5.5, 95°C Pyrococcus furiosus
19.8
-
narirutin pH 5.5, 95°C Pyrococcus furiosus
42.1
-
hesperidin pH 5.5, 95°C Pyrococcus furiosus