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Literature summary for 3.2.1.B28 extracted from
- Purification and characterization of an extremely thermostable beta-glucosidase from the hyperthermophilic archaeon Pyrococcus furiosus (1993), Eur. J. Biochem., 213, 305-312.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-glucose | weak | Pyrococcus furiosus |  |
| SDS | 0.1%, activity is completely and irreversibly blocked | Pyrococcus furiosus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.15 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5, 80°C | Pyrococcus furiosus | |
| 20 | - |
cellobiose | pH 5, 80°C | Pyrococcus furiosus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Pyrococcus furiosus | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | divalent metal ions not required | Pyrococcus furiosus | |
| NaCl | optimal concentration: 200 mM | Pyrococcus furiosus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
4 * 58000, SDD-PAGE | Pyrococcus furiosus |
| 230000 | - |
non-denaturing PAGE | Pyrococcus furiosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pyrococcus furiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-galactopyranoside + H2O | hydrolysed at 34% compared to activity with 4-nitrophenyl-beta-D-glucopyranoside | Pyrococcus furiosus | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Pyrococcus furiosus | 4-nitrophenol + D-glucopyranose | - |
? | |
| 4-nitrophenyl beta-D-xylopyranoside + H2O | hydrolysed at 9.2% compared to activity with 4-nitrophenyl-beta-D-glucopyranoside | Pyrococcus furiosus | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
| cellobiose + H2O | as active as 4-nitrophenyl-beta-D-glucopyranoside | Pyrococcus furiosus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 58000, SDD-PAGE | Pyrococcus furiosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 102 | 105 | - |
Pyrococcus furiosus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | 110 | 80°C: about 45% of maximal activity, 110°C: about 65% of maximal activity | Pyrococcus furiosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
half-life: 85 h | Pyrococcus furiosus |
| 110 | - |
half-life: 13 h | Pyrococcus furiosus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 454 | - |
cellobiose | pH 5, 80°C | Pyrococcus furiosus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Pyrococcus furiosus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 300 | - |
D-glucose | pH 5, 80°C | Pyrococcus furiosus | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pyrococcus furiosus | isoelectric focusing | - |
4.4 |
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